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Analytical Chemistry and Chromatography Techniques

Running You Through HPLC: How Does it Work?

Your advisor tells you that he wants you to use HPLC to analyze your compound. You know you’ve heard of this technique before, but you can’t remember what HPLC stands for, let alone how to go about doing it! We’ve all been there, and I bet you wish you had paid more attention in that…

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Exploiting HPLC and UHPLC Selectivity With Rational Stationary Phase Design

In this seminar, we explore the power of selectivity using the resolution equation in HPLC and UHPLC. Using a variety of chromatographic data, we discuss how incorporating an aromatic building block in phase design has led to the unique bonded phases ACE® C18-AR and ACE® C18-PFP. These unique phases have been engineered based upon the popular…

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What Can NMR Do For You? — Part Two

Welcome to part two of “What Can NMR Do For You?”, a three-part series in which we see how you can use simple NMR experiments in your research. In part one, we went over some key points to keep in mind when doing NMR on proteins and DNA, such as sample preparation, and saw how…

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What Can NMR Do For You? – Part One

Having done my PhD work in an NMR lab, I sometimes feel like NMR doesn’t get the credit it deserves. As a structural tool, it always seems to play second fiddle to crystallography, and not many people seem to know about all of the different types of information that NMR can give you.> So why…

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An Introduction to Tandem Affinity Purification

Tandem affinity purification is a development of existing techniques for purifying protein complexes from cells in physiological conditions. It was first described over ten years ago and has become a commonplace laboratory tool. In this brief article I’ll introduce the basic technique and describe some of its advantages. Biology is a team game. Most biological…

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Fast separation of peptides using conventional HPLC equipment

Dr Barry Boyes of, Director of Bioscience R&D for Advanced Materials Technologies, Inc, will present a guide to using conventional HPLC equipment to separate peptides. The past decade has shown considerable progress in the development of materials and methods for high speed and high resolution separations of peptides and proteins. Improvements in instrumentation have certainly…

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