Analytical Chemistry and Chromatography Techniques
Running You Through HPLC: How Does it Work?
Your advisor tells you that he wants you to use HPLC to analyze your compound. You know you’ve heard of this technique before, but you can’t remember what HPLC stands for, let alone how to go about doing it! We’ve all been there, and I bet you wish you had paid more attention in that…
Read More Exploiting HPLC and UHPLC Selectivity With Rational Stationary Phase Design
In this seminar, we explore the power of selectivity using the resolution equation in HPLC and UHPLC. Using a variety of chromatographic data, we discuss how incorporating an aromatic building block in phase design has led to the unique bonded phases ACE® C18-AR and ACE® C18-PFP. These unique phases have been engineered based upon the popular…
Read More What Can NMR Do For You? — Part Two
Welcome to part two of “What Can NMR Do For You?”, a three-part series in which we see how you can use simple NMR experiments in your research. In part one, we went over some key points to keep in mind when doing NMR on proteins and DNA, such as sample preparation, and saw how…
Read More Recent Developments in Fused-Core Technology for the High Speed High Resolution Analysis of Biomolecules
HPLC separations of biomolecules have traditionally required much time in order to achieve the required resolution for complex samples. However, this has recently begun to change with the developments that have been made in Fused-Core particle technology. HPLC columns of high purity silica that are 2.7 µm in total particle size with a 0.5 µm…
Read More What Can NMR Do For You? – Part One
Having done my PhD work in an NMR lab, I sometimes feel like NMR doesn’t get the credit it deserves. As a structural tool, it always seems to play second fiddle to crystallography, and not many people seem to know about all of the different types of information that NMR can give you.> So why…
Read More An Introduction to Tandem Affinity Purification
Tandem affinity purification is a development of existing techniques for purifying protein complexes from cells in physiological conditions. It was first described over ten years ago and has become a commonplace laboratory tool. In this brief article I’ll introduce the basic technique and describe some of its advantages. Biology is a team game. Most biological…
Read More Fast separation of peptides using conventional HPLC equipment
Dr Barry Boyes of, Director of Bioscience R&D for Advanced Materials Technologies, Inc, will present a guide to using conventional HPLC equipment to separate peptides. The past decade has shown considerable progress in the development of materials and methods for high speed and high resolution separations of peptides and proteins. Improvements in instrumentation have certainly…
Read More Applying Spectroscopy Techniques to Biology: Cracking the cause of Japanese beetle paralysis
An article in the latest issue of Proceedings of the Natural Academy of Sciences (PNAS) caught my eye, which showcases quite nicely that a multitude of chemical spectroscopy techniques can be used for solving a simple observation that dates back nearly 100 years! In short, a team of US scientists using high-resolution mass spectrometry and…
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