Quantcast
Skip to content

Sigma-Aldrich® Advanced Genomics

What’s that Organism? Using DNA Barcoding for Species Identification

In both the lab and field, it is important to know what species we are working with. While morphological data has always been a tried and true method of identifying species, DNA barcoding allows us to identify species when we don’t have that option (e.g. if we don’t have enough of a specimen to identify…

DNA barcodingRead More

Kick-Start Your Gut Microbiome Study in Four Easy Steps

Today, the gut microbiome is garnering a large amount of media attention for its role in human health and disease. From influencing immune responses to impact our brain, the gut microbiome is an important and necessary aspect of our life. So much so, that current investigations in the gut microbiome are focusing on developing biomarkers for…

gut microbiomeRead More

Studying the Epigenome by Next Generation Sequencing

The epigenome has been in the research spotlight, and for good reason. Not only has it been associated with the developmental stages of an organism, but epigenetic alterations lead to disorders and have been linked to many human diseases. So, the question stands: what exactly is an epigenome? What Is the Epigenome? Simply put, the…

DNA sequenceRead More

Using dbSNP and ClinVar to Classify Gene Variants

As we discussed previously, the gaps in our understanding of the human genome make variant classification an extremely difficult job. However, with each passing day our knowledge increases, and the tools to help us become increasingly more efficient. Let’s pick up where we left off in our first article about variants. After checking Ensemble to…

Read More

Discovering PARP inhibitor resistance mechanisms using genome-wide and focused CRISPR screens

Discovering PARP inhibitor resistance mechanisms using genome-wide and focused CRISPR screens In this webinar, Dr. Stephen Pettitt explains how he applies genome-wide targeted mutagenesis screens to elucidate the genetic basis of drug resistance. Using mouse and breast cancer cell lines, Dr. Pettitt’s team developed a targeted, genome-wide mutagenesis screen to identify mutations responsible for resistance…

DNA strandsRead More

Common challenges and solutions in microbiome analysis workflows

Common challenges and solutions in microbiome analysis workflows Elucidating meaningful, unbiased microbial community profiles from complex microbiome samples is challenging.In this tutorial, you will find: the sources of bias throughout the microbiome analysis workflow practical solutions for troubleshooting your techniques new technologies to achieve the most representative and unbiased microbiome profiles Speakers Sven Reister, Ph.D.PCR…

Read More

A Beginner’s Guide to Single-Cell RNA Sequencing

Since our early steps in Science we have been told that every cell in our body has the exact same genetic information (minus one or two alterations). Therefore, the great variety of cells in our body comes from gene expression – each cell must express one set of genes and repress another set to function…

Small child hiding in hallway, playing hide and seekRead More

Simultaneous Proteomics and Transcriptomics: The Future of Single Cell Analysis

Simultaneous Proteomics and Transcriptomics: The Future of Single Cell Analysis Join us in this webinar featuring Dr. Marlon Stoeckius as he explains how you can improve your single-cell RNA-sequencing (scRNA-seq) experiments.In this tutorial, you will find: How you can run one scRNA-seq experiment with numerous protein markers in parallel How you can increase your recovery of…

Image showing conjugated binding and not binding with RNARead More

Long Adapter Single Stranded Oligonucleotide (LASSO) Probes for Massively Multiplexed Cloning of Kilobase-Sized Genome Regions

In this webinar, you will learn how to solve a major problem in creating expression libraries from genome sequences for downstream analyses. Specifically, you will learn: The difference and benefits of LASSO cloning over NGS How LASSO cloning allows for the multiplex cloning of large open reading frames (ORFs) of bacterial, human, and human-microbiome genomes…

PCRRead More

De Novo DNA Sequencing and the Special k-mer

The technology for DNA sequencing was developed back in 1977 thanks to Frederick Sanger. It took a bit longer before it was possible to sequence a complete genome. This is because we needed an appropriate mathematical model and massive computational power to assemble millions or billions of small reads to a larger complete genome. Today’s…

K-merRead More

How to Become a Bioinformatician

If you want to start a journey towards being a bioinformatician, you must first know the meaning of the term bioinformatics. You will also need to know what bioinformaticians do, and what you need to know to land your first entry level job as a bioinformatician. Most bioinformaticians work within different medical science and health…

bioinformaticsRead More

A Crash Course in BLAST Searching

Simple BLAST searching is pretty straightforward to many of us. Just plug in your sequence, select the species genome, and hit search! But have you ever wondered what it takes to run a BLAST query using these mammoth-sized (no pun intended!) sequence databases? BLAST searching can produce dozens, hundreds, or even thousands of candidate alignments.…

Read More

How to Use Ensembl to Classify Gene Variants

Even though our knowledge about genomes grows daily, and in huge leaps, we sometimes need to remind ourselves that DNA was first isolated in 1869 and its molecular structure was only identified in 1953. The PCR reaction only hit the scientific community as recently as 1983! So even though we are growing fast, we are…

Read More

Understanding and Designing Flanking Homology DNA Assembly Experiments

Understanding and Designing Flanking Homology DNA Assembly Experiments Join us in this webinar on demystifying DNA assembly.In this tutorial, you will find: How flanking homology DNA assembly methods work How to use web-based software to design experimental methods for flanking homology DNA assembly methods How synthetic DNA fragments fit in to the DNA assembly process…

Read More

Why You Should Use Cas9 Ribonucleoprotein Transformation for CRISPR Genome Editing

Imagine directly creating a mutation at (almost) any site in your target genome instead of screening thousands or millions of random mutants! The CRISPR/Cas9 system does just that. In its traditional form, this forward genetics approach takes 7 steps from start to mutated genome. However, there is a way to obtain your designer genome in…

ribonucleoprotein transformationRead More

The Good, the Bad and the Expensive of Whole Genome Sequencing

Whole Genome Sequencing (WGS) is still very cutting edge, sequencing technology and while there are a lot of perks to using it, there are also a few drawbacks. The good, the bad and the pricey are outlined below to help you navigate when it’s worth using WGS! Whole Genome Sequencing: The Good Lots of Data…

whole genome sequencingRead More

Next Gen Sequencing Challenges

Next generation sequencing opened the doors to our genome. It gives massive amounts of information in a week – whereas Sanger sequencing takes thrice as long, and causes lab lesions due to the abusive use of pipettes. Indeed, with minimal hands-on procedures we obtain a lot of data. But nothing in Science is ever easy.…

Read More
Scroll To Top