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Level Up Your Drug Screening With CRISPR

…pooled versus arrayed approaches. © 2015 Agrotis and Ketteler [5], licensed under CC BY 4.0. MOI, multiplicity of infection; sgRNA, single guide RNA. Pooled Library Screens A pooled library can…

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A Primer on Phage Display Libraries

…In a semi-synthetic library, these framework regions are amplified and cloned into the phage display library from B-cells, in differing combinations to achieve different goals. In a synthetic library, these…

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8 Approaches to Random Mutagenesis

…then randomly introduce mutations into the gene sequence to create a “library” containing thousands of versions of the gene. Each version (or “variant”) of the gene in the library would…

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How to Improve Your WGS DNA Library

Library Prep Kit for Illumina Systems and Invitrogen™ Collibri™ PS DNA Library Prep Kit for Illumina Systems, it is possible to reduce these biases. References Macintyre G, Yistra B, and…

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From the Lab to the Library…

…did indeed go back to school to get my Masters in Library Science (MLS).Then I looked at library and university online job sites to look for openings (check out the…

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Quantifying Your NGS Libraries

…very high-throughput. Illumina recommends the very capable KAPA library quantification kit, but there are alternatives including Agilent, Qiagen and QuantBio, among others. The KAPA kit is a SYBR-green assay that…

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A Colorful Route to Sequencing Success

…Collibri PS DNA Library Prep Kit for Illumina systems provide visual feedback that allows real-time tracking of the successful mixing of reagents at each step in the library preparation and…

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Generating RNA-seq Libraries from RNA

…starts with size selection for small RNAs, ligation of selected fragments to adapters, and then generation of cDNA.4 New RNA Library Preparation Method A new RNA library prep method creates…

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An Introduction to RNA-seq

library prep which includes end-repair, adapter ligation and PCR amplification to give you a library ready for sequencing. Why Bother With Strand Information? There has been a lot of discussion…

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Probability Theory and Molecular Barcodes

…As an example, this approach enables parallel sequencing of different samples through the labeling of each library with a distinguishing barcode, or index (e.g. Illumina indexing). The procedure is straightforward:…

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Best Practices for DNA Shearing for NGS

Construction of high-quality sequencing libraries is pivotal to successful NGS, and DNA quality is one of the most critical aspects of library preparation. As this Nature Methods paper illustrates, DNA…

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