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Vicki Doronina

Vicki did her PhD in Molecular Biology at the University of Edinburgh. She had been working as a postdoc in several Russel group UK universities, while honing her skills in scientific and creative writing. She is now a pen for hire. Check out my proudest achievement, which may be useful for you: The BiteSizeBio Guide for Protein expression

Articles by Vicki Doronina:

The Sandwich Technique: How to Dish Out Critique

How time flies. One day you are giving your first presentation in front of your fellow students, seemingly the next you are listening to a presentation by your own student. And frankly, the talk is awful. It’s twice the time limit, the student weaves around the topic as a drunken sailor, and she acknowledges her…

19 Jul 2017 Dealing with Fellow Scientists

Top 5 of the most commonly used cell lines!

Cell lines are an invaluable scientific tool. They allow us to dissect the internal workings of tissues in a controlled environment without the ethical implications of working with whole organisms. Starting with the first successful immortal cell line HeLa, the number of available cell lines has since diversified into a plethora of options. Just like model…

13 Apr 2017 Cells and Model Organisms

Setting up a Fermentation or Perpetuum Mobile Cell Culture

Some names are confusing. For example, ant-lion is not an ant – or a lion. Likewise, fermentation in the scientific sense does not involve using a ferment or brewing beer. In science, fermentation is the setting up of a long-term culture of eukaryotic or prokaryotic cells. Fermentation is invaluable in providing a steady flow of…

10 Apr 2017 Cells and Model Organisms

How to Use Proteases to Purposefully Digest Proteins

In this article I will not talk about ‘wild’ proteases, which destroy cellular proteins in your lysates like wolves destroy sheep. Instead, I’ll be talking about the shepherd dog proteases—purified, tame and useful to digest proteins your research. In Protein Research and Crystallization Several programs can predict your protein domains. However, we wet biologists know…

25 Aug 2016 Protein Expression and Analysis

How to Improve Plasmid Yield Using Antibiotics

After the initial excitement of growing and isolating plasmid DNA, routinely preparing plasmid mini/midi/maxi preps gets boring. You definitely want a way to squeeze the maximum amount of plasmid DNA out of your culture. Good news—you can increase your plasmid yield using antibiotics. Keep the Pressure On to Improve Plasmid Yield Remember that you need…

23 Aug 2016 DNA / RNA Manipulation and Analysis
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