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Victoria Doronina

Vicki did her PhD in Molecular Biology at the University of Edinburgh. She had been working as a postdoc in several Russel group UK universities while honing her skills in scientific and creative writing. She is now a Technical Officer at the Manchester Metropolitan University, UK, and pen for hire. Check out my proudest achievement, which may be useful for you: The BiteSizeBio Guide for Protein expression

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Articles by Victoria Doronina

An image of different sized leaves to depict kit-free midiprep.

How to Do a Kit-free Midiprep

By Vicki Doronina | July 23, 2012

Here, we share a protocol for a midiprep, which, if not faster, gives a larger plasmid DNA yield than any commercial midiprep kit.

Got Phage? Here's how to get rid of it.

Got Phage? Here’s how to get rid of it.

By Vicki Doronina | July 18, 2012

Summertime… The birds are singing, the trees are growing. Your tissue culture has sprouted yeast contamination, your yeast culture is happily growing bacteria. Your bacterial culture was growing calmly and predictably, dividing every twenty minutes, but suddenly its optical density has dropped, and it’s full of some sort of filaments and clumps. Or you did…

How to Be The Lab Bastard

How to Be The Lab Bastard

By Vicki Doronina | July 3, 2012

We all know them. You might even be one. The Lab Bastard is the one who considers himself (or herself!) superior to all other mere mortals in the lab. He would never degrade his talent by doing communal jobs in the lab, but swans around, absolutely sure that his experiments are most important and his…

The Easiest Yeast Transformation Protocol on Earth

The Easiest Yeast Transformation Protocol on Earth

By Vicki Doronina | June 22, 2012

There are several yeast transformation protocols around, and most of them require a lot of steps: overnight starter culture, dilution and growth to logarithmic phase, several washes, and so on… These protocols work very well since they have been optimised for maximum transformation efficiency, which is needed for applications like library construction. But they are…

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