Victoria Doronina
Vicki did her PhD in Molecular Biology at the University of Edinburgh. She had been working as a postdoc in several Russel group UK universities while honing her skills in scientific and creative writing. She is now a Technical Officer at the Manchester Metropolitan University, UK, and pen for hire. Check out my proudest achievement, which may be useful for you: The BiteSizeBio Guide for Protein expression
Articles by Victoria Doronina
Here, we share a protocol for a midiprep, which, if not faster, gives a larger plasmid DNA yield than any commercial midiprep kit.
Summertime… The birds are singing, the trees are growing. Your tissue culture has sprouted yeast contamination, your yeast culture is happily growing bacteria. Your bacterial culture was growing calmly and predictably, dividing every twenty minutes, but suddenly its optical density has dropped, and it’s full of some sort of filaments and clumps. Or you did…
We all know them. You might even be one. The Lab Bastard is the one who considers himself (or herself!) superior to all other mere mortals in the lab. He would never degrade his talent by doing communal jobs in the lab, but swans around, absolutely sure that his experiments are most important and his…
There are several yeast transformation protocols around, and most of them require a lot of steps: overnight starter culture, dilution and growth to logarithmic phase, several washes, and so on… These protocols work very well since they have been optimised for maximum transformation efficiency, which is needed for applications like library construction. But they are…