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Francesc Codony

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Life of the Party: Viability PCR (vPCR)

Life of the Party: Viability PCR (vPCR)

By Francesc Codony | July 9, 2016

Viability PCR (vPCR) is a big step forward in PCR technology. Through the use of a simple pre-treatment of the sample(s) of interest using specific intercalating reagents, it is possible to neutralize the DNA of dead cells. As a result, only DNA from live cells will be amplified by PCR. Through the vPCR, it’s possible to…

Loop-mediated Isothermal Amplification

Loop-mediated Isothermal Amplification

By Francesc Codony | February 16, 2015

Loop-mediated Isothermal amplification (LAMP), is an emerging technology that allows DNA amplification at a constant temperature. The key to this principle is the use of a DNA polymerase that possesses strand displacement activity. As a result of this property there is no need for heat denaturation of double stranded DNA in order to allow primer…

Do-it-Yourself PCR

Do-it-Yourself PCR

By Francesc Codony | July 17, 2014

Currently Open Source principles are offering interesting tools for doing molecular biology at an incredibly low cost. One interesting example is OpenPCR (www.openPCR.org) a project developed in order to ensure that the basic technology to perform PCR is affordably and openly available to all. In the past one of the main barriers for introducing PCR technology…

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