Tim Bushnell

I am a technical applications scientist, who's job is to help researchers get the best possible data out of their experiments as possible. As director of research cores, it is my job to ensure that these research cores operate at the highest level to support the research of the URMC investigators while being fiscally responsible and responsive. I also run the concierge cytometry company, Expert Cytometry (www.expertcytometry.com). Our focus is to provide training and education to researchers around the globe so they can maximize their return on their cytometry experiments. Specialties: Flow Cytometry, design of polychromatic panels, data analysis, quality control, core management operation, training, education

Articles by Tim Bushnell:

Introducing CyTOF: Cytometry of the Masses

Flow cytometry remains unparalleled as a single-cell analysis technology.  The ability to analyze 14 or more fluorescent parameters on a million cells or more allows for detailed understanding of complex biological processes. The Problem With Traditional Flow Cytometry One limitation of flow cytometry is the reliance on fluorescent tags.   Even with careful panel design, loss…

19 Jul 2016 Flow Cytometry

An Introduction to Spectral Overlap and Compensation Protocols in Flow Cytometry

It strikes fear into the hearts of new cytometrists. Compensation. More fights have started over the proper way to compensate at meetings than anything else. This article will strive to shed some light on the principles of compensation, and equip you with the tools necessary to achieve compensation mastery for your research experiments. Compensation is…

09 Jul 2016 Flow Cytometry

Setting Voltages for Optimal Sensitivity

After panel design, and titration of the reagents, the next most important step in flow cytometry is setting the proper voltages on the photomultipler tubes (PMTs). These detectors take the photons of light emitted by the fluorochromes on the cells and convert them to electrons, which ultimately become the voltage current that is digitized and…

02 Jun 2015 Flow Cytometry

Importance of Antibody Titration in Flow cytometry

After designing a multicolor flow cytometry panel and securing the necessary cells and reagents, the process of optimization of the panel can begin. The first step in that optimization is titration of your antibodies. In this process, following a standard protocol to be used in the final analysis, you stain a known amount of cells with…

17 Feb 2015 Flow Cytometry

Beginners Guide to Designing Your Own Polychromatic Flow Panel

“The greater the power, the more dangerous the abuse.” – Edmund Burke “With great power comes great responsibility.” – Uncle Ben (quoting Voltaire) While it’s unlikely that either of these speakers performed multi-dimensional flow cytometry, it is important to remember these quotes in the context of developing and implementing a good polychromatic flow panel.  More fluorochromes are…

03 Feb 2015 Flow Cytometry