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Sylvia Norman

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Mediator Probe PCR

Mediator Probe PCR

By Sylvia Norman | November 6, 2014

Want a PCR method that gives you high sensitivity and is cost-effective? Then you might want to try Mediator Probe PCR.  This method gives you sensitivity and limit of detection comparable to that of dual-labeled hydrolysis probes without the high cost. We all know how expensive small batch probe synthesis orders can be, especially when…

MIQE Guidelines for Digital PCR

MIQE Guidelines for Digital PCR

By Sylvia Norman | September 4, 2014

MIQE what’s that? When writing dPCR materials and methods for a paper have you ever pondered what information you should include? This is where the MIQE guidelines will really help. Guidelines for minimum information required for publication of a digital PCR (dPCR) experiment were published by JF Huggett et al. in 2013. These were a…

Digital PCR or Quantitative Real-Time PCR: Which Method Is Best for Your Quantitative PCR Application?

Digital PCR or Quantitative Real-Time PCR: Which Method Is Best for Your Quantitative PCR Application?

By Sylvia Norman | May 1, 2014

So you’re designing a new experiment that requires PCR quantification. You used to have only one method to choose from, but now you have two – Quantitative Real-Time PCR (qPCR) and Digital PCR (dPCR). Which one is right for your application? Both methods have good quantification, sensitivity and specificity for most applications. They are compatible…

What is Digital PCR?

What is Digital PCR?

By Sylvia Norman | March 6, 2014

Digital PCR (dPCR) is a quantitative PCR method that provides a sensitive and reproducible way of measuring the amount of DNA or RNA present in a sample. This method is similar to qPCR in the reaction assembly components and amplification reaction, but differs in the way the sample target is measured.  Digital PCR is a…

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