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Rolly U. Wiegand

Rolly is Head of the Confocal and Advanced Light Microscopy (CALM) facility in the College for Medicine and Veterinary Medicine at the University of Edinburgh. In this function he also co-ordinates the strategic planning for various light microscopy-related projects across the College.

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Articles by Rolly U. Wiegand

A Microscopy Glossary Part 2: ‘Confocality Means….’

A Microscopy Glossary Part 2: ‘Confocality Means….’

By Rolly U. Wiegand | September 25, 2012

Dichroic Mirror/Filter This is a semi-reflective filter which can also be referred to as ‘dichromatic beam splitter’. Unlike the Longpass filters which absorb light which is not transmitted (see Part 1 of the Glossary), these filters reflect light at lower wavelengths and transmit light at wavelengths above the ‘cut-on’ wavelength. As beam splitters, they are…

A Microscopy Glossary Part 1: ‘What Is This LP650 You Talk About?!’

A Microscopy Glossary Part 1: ‘What Is This LP650 You Talk About?!’

By Rolly U. Wiegand | September 18, 2012

Brightfield Illumination This defines the most basic method of optical microscopy using white light to illuminate the sample in the transmitted mode. Absorption and diffraction of the light by the molecules in the specimen generates the contrast in the image. Methods such as darkfield illumination, differential interference contrast and phase contrast help to increase the…

Which Light Microscope Will I Choose? Part 2- Confocal Systems

By Rolly U. Wiegand | September 4, 2012

Why confocal? The standard fluorescence widefield microscope described in our last article has one major disadvantage: it collects not only the desired image information from the focal plane, but it also records a large amount of out-of-focus light, leading to a blurred image. In the 1950’s, system developers started thinking about how to get rid…

Which Light Microscope Will I Choose? Part 1 – Basic Light and Fluorescence Systems

By Rolly U. Wiegand | August 28, 2012

Think before you start! Before you even start preparing your samples, you should think about the choice of microscope for image acquisition. Manufacturers offer an ever increasing range of light microscopes- some of which may already be available in your research institutes. To help you get the best out of your imaging experiment by making the…

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