Olwen Reina

I am a Clinical Research Coordinator at the U.S. Department of Veterans Affairs with a background in basic research, writing, mentoring and teaching. I studied Natural Science at Trinity College Dublin, Ireland, specializing in biochemistry with immunology and I am currently undergoing ACRP (Association of Clinical Research Professionals) certification. In my spare time, I enjoy studying HTML/CSS and SEO, doing acroyoga, making kombucha, salsa dancing, voluntary community projects and eating sushi. Feel free to send me a note with any writing opportunities or to say hello.

Articles by Olwen Reina:

When PCR Gets RACE-y: From Unknown mRNA Segments to Sequenced cDNA

Normally you need two primers to amplify your segment of interest – one for the 3′ end of your segment of interest and one for your 5′ end. But if you don’t know the sequence of the regions you’re hoping to amplify this can be a problem! Rapid Amplification of cDNA Ends (RACE) is a…

09 Jul 2016 PCR, qPCR and qRT-PCR

Top 10 Tips for Phenol-Chloroform Extractions and Ethanol Precipitations

Phenol-chloroform extractions and ethanol precipitations can be a royal pain, but here are some tips to help you get tip-top results!

09 Jul 2016 DNA / RNA Manipulation and Analysis

3 Carcinoma Cells, 2 Stromal Cells and a Partridge in a Pear Tree: An Introduction to Cell Co-Culture

The first thing you learn about culturing cells is proper aseptic technique and avoiding contamination. After that you’ll learn all the ins and outs of culturing your project’s specific cell line(s). What may not have been covered, is co-culturing, and I don’t just mean ethnic diversity in the lab! Co-culturing is the indirect or direct…

09 Jul 2016 Cells and Model Organisms

Native Versus Denaturing Gels

We’re already gone through the basics of how gel electrophoresis work, compared common gel types like agarose and polyacrylamide and even explored some alternatives. Now let’s look at the native versus denaturing gels. You’ll be a speGEList in no time! Denaturing Gels We’ll start with this one, as it’s very self-explanatory. Denaturing gels are exactly…

09 Jul 2016 Protein Expression and Analysis

Time to Instigate Nested PCR

How to Obtain a Purer PCR Product and Reduce Non-specific Amplification Unless you’ve gotten your hands on some miraculously specific primers, amplification of only your target sequence without non-specific amplification can be very challenging. Thankfully, a clever and surprisingly simple solution is at hand! A Quick Recap of the Basics In PCR, you design your…

09 Jul 2016 PCR, qPCR and qRT-PCR
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