Articles by Patrick Miller-Rhodes:
If you need a multi-gene knockout or large-scale genomic modification, or want reduced off-target effects, then multiplex CRISPR is for you!
Are needing to choose a microglial cell line? Want to ensure you are currently using the right one for your needs? Discover the different options available and how to choose.
If you’re confused about what N numbers mean for cell lines, you’re not alone. We explain it all and give you a guide on best practice.
Want to increase siRNA stability and efficiency? Read on to learn five chemical modifications that will help.
Get your data out here! Learn about several venues that publish solid data, even if they don’t support your hypothesis or tell a story.
Discover two CRISPR-based viral diagnostic strategies, DETECTR and SHERLOCK.
Confused about CRISPR nucleases? Read this guide to discover the various CRISPR nucleases available and what they are best suited for.
Although multiplex CRISPR gene editing can be accomplished by simply introducing more than one gRNA to your target cells, there are many alternative — and more efficient — ways of achieving this goal. This article discusses these alternative CRISPR multiplexing strategies and highlights their potential caveats. Not sure whether multiplex CRISPR gene editing is right…
Overnight ligations are inconvenient — especially when they fail. Luckily, there’s a straightforward way to faster DNA ligations. This article highlights the secret ingredient to faster ligation reactions and offers some tips and caveats on its use. For a general overview of DNA ligations, see here and here. Buy a Quick Ligation Kit The most…
How to Prevent False Results in Colony PCR Colony PCR saves time and reduces costs by eliminating the need for plasmid purification. However, confounding results abound — but only if you fail to anticipate them. This article outlines the major perpetrators of false results and how to prevent them. For a more general overview of…
CRISPRa allows you to activate or overexpress genes in a more endogenous manner. Find out the steps to getting started.