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Patrick Miller-Rhodes

Patrick has a PhD in Neuroscience from the University of Rochester. He is a Science Marketing Writer.

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Articles by Patrick Miller-Rhodes

Image of amultiplex theatre to represent multiplex CRISPR experiments

Multiplex CRISPR Gene Editing: What You Need to Know

By Patrick Miller-Rhodes | May 27, 2021

If you need a multi-gene knockout or large-scale genomic modification, or want reduced off-target effects, then multiplex CRISPR is for you!

image of microglia

Microglial Cell Lines: The Ultimate Guide to Selection

By Patrick Miller-Rhodes | September 2, 2020

Are needing to choose a microglial cell line? Want to ensure you are currently using the right one for your needs? Discover the different options available and how to choose.

Woman holding numebr 3 looking confused, representing how unclear n numbers for cell line experiments are

What Does “N” Mean When Using Cell Lines?

By Patrick Miller-Rhodes | July 7, 2020

If you’re confused about what N numbers mean for cell lines, you’re not alone. We explain it all and give you a guide on best practice.

Five repairmen working denoting five modifications

Five Ways to Modify Your siRNA for Improved RNAi 

By Patrick Miller-Rhodes | June 4, 2020

Want to increase siRNA stability and efficiency? Read on to learn five chemical modifications that will help.

Man holding papers representing all work to be published

How to Publish Negative Results (And Everything Else in Your Lab Notebook) 

By Patrick Miller-Rhodes | May 28, 2020

Get your data out here! Learn about several venues that publish solid data, even if they don’t support your hypothesis or tell a story.

A detective dressed like a detective to symbolize the DETECTR and SHERLOCK CRISPR methods for Viral diagnosis.

How CRISPR Can Be Used to Detect Emerging Viral Pathogens

By Patrick Miller-Rhodes | May 19, 2020

Discover two CRISPR-based viral diagnostic strategies, DETECTR and SHERLOCK.

CRISPR Nucleases: The Ultimate Guide To Selection

CRISPR Nucleases: The Ultimate Guide To Selection

By Patrick Miller-Rhodes | February 4, 2020

Confused about CRISPR nucleases? Read this guide to discover the various CRISPR nucleases available and what they are best suited for.

CRISPR Multiplexing Strategies: What’s The Choice?

CRISPR Multiplexing Strategies: What’s The Choice?

By Patrick Miller-Rhodes | January 28, 2020

Although multiplex CRISPR gene editing can be accomplished by simply introducing more than one gRNA to your target cells, there are many alternative — and more efficient — ways of achieving this goal. This article discusses these alternative CRISPR multiplexing strategies and highlights their potential caveats. Not sure whether multiplex CRISPR gene editing is right…

An graphic of expanding circles to depict a method for cheaper bacterial transformation.

Faster Ligations: PEGing down the Secret

By Patrick Miller-Rhodes | November 27, 2019

Overnight ligations are inconvenient — especially when they fail. Luckily, there’s a straightforward way to faster DNA ligations. This article highlights the secret ingredient to faster ligation reactions and offers some tips and caveats on its use. For a general overview of DNA ligations, see here and here. Buy a Quick Ligation Kit The most…

How to Prevent False Results in Colony PCR

How to Prevent False Results in Colony PCR

By Patrick Miller-Rhodes | October 17, 2019

How to Prevent False Results in Colony PCR Colony PCR saves time and reduces costs by eliminating the need for plasmid purification. However, confounding results abound — but only if you fail to anticipate them. This article outlines the major perpetrators of false results and how to prevent them. For a more general overview of…

A light switch depiciting how CRISPRa allows you to swtich gene expression on

CRISPR-Based Activation (CRISPRa): A How-To Guide

By Patrick Miller-Rhodes | October 4, 2019

CRISPRa allows you to activate or overexpress genes in a more endogenous manner. Find out the steps to getting started.

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