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Patrick Miller-Rhodes

"Patrick is equal parts neuroscientist, writer, and extreme sports enthusiast. He is currently a Ph.D. candidate at the University of Rochester Medical Center, where he uses molecular biology techniques to study inflammation and neurodegeneration. To flesh out his scientific and medical writing portfolio, he pursues freelance writing opportunities when he’s not at the bench or off the grid.

Articles by Patrick Miller-Rhodes:

Microglial Cell Lines: The Ultimate Guide to Selection

Are needing to choose a microglial cell line? Want to ensure you are currently using the right one for your needs? Discover the different options available and how to choose.

image of microglia
02 Sep 2020 Cell Culture

What Does “N” Mean When Using Cell Lines?

If you’re confused about what N numbers mean for cell lines, you’re not alone. We explain it all and give you a guide on best practice.

Woman holding numebr 3 looking confused, representing how unclear n numbers for cell line experiments are
07 Jul 2020 Cells and Model Organisms

Five Ways to Modify Your siRNA for Improved RNAi 

Want to increase siRNA stability and efficiency? Read on to learn five chemical modifications that will help.

Five repairmen working denoting five modifications
04 Jun 2020 Chemistry for Biologists&DNA / RNA Manipulation and Analysis

How to Publish Negative Results (And Everything Else in Your Lab Notebook) 

Get your data out here! Learn about several venues that publish solid data, even if they don’t support your hypothesis or tell a story.

Man holding papers representing all work to be published
28 May 2020 Taming the Literature&Writing, Publishing and Presenting

How CRISPR Can Be Used to Detect Emerging Viral Pathogens

The threat of pandemic viruses can be mitigated by developing diagnostic tests that are rapidly implementable and field deployable. Two CRISPR-based strategies, DETECTR and SHERLOCK, have been developed recently that not only meet these criteria but offer unprecedented multiplexing capabilities. This article discusses the theoretical basis of proposed and currently available viral diagnostics for educational…

A detective dressed like a detective to symbolize the DETECTR and SHERLOCK CRISPR methods for Viral diagnosis.
19 May 2020 Genomics and Epigenetics

CRISPR Nucleases: The Ultimate Guide To Selection

CRISPR nucleases continue to dominate the gene-editing landscape because they are powerful and easy-to-use gene-editing tools. Their rapid discovery and development, however, make choosing the right nuclease for your experiment difficult. This guide highlights the broad range of available CRISPR nucleases and distils the major factors you should consider when choosing one for your next…

04 Feb 2020 Genomics and Epigenetics&Sigma-Aldrich® Advanced Genomics

CRISPR Multiplexing Strategies: What’s The Choice?

Although multiplex CRISPR gene editing can be accomplished by simply introducing more than one gRNA to your target cells, there are many alternative — and more efficient — ways of achieving this goal. This article discusses these alternative CRISPR multiplexing strategies and highlights their potential caveats. Not sure whether multiplex CRISPR gene editing is right…

28 Jan 2020 Genomics and Epigenetics&Sigma-Aldrich® Advanced Genomics

Multiplex CRISPR Gene Editing: What’s It For?

The RNA-guided nature of CRISPR nucleases (e.g., Cas9, Cpf1) makes them highly amenable to multiplex applications, including multi-gene knockout and large-scale genomic modifications. This article — part 1 of a two-part series on multiplex CRISPR gene editing — discusses these applications and outlines practical considerations for multiplex CRISPR gene editing experiments. New to gene editing…

21 Jan 2020 Genomics and Epigenetics&Sigma-Aldrich® Advanced Genomics

Faster Ligations: PEGing down the Secret

Overnight ligations are inconvenient — especially when they fail. Luckily, there’s a straightforward way to faster DNA ligations. This article highlights the secret ingredient to faster ligation reactions and offers some tips and caveats on its use. For a general overview of DNA ligations, see here and here. Buy a Quick Ligation Kit The most…

27 Nov 2019 DNA / RNA Manipulation and Analysis&New England Biolabs

How to Prevent False Results in Colony PCR

How to Prevent False Results in Colony PCR Colony PCR saves time and reduces costs by eliminating the need for plasmid purification. However, confounding results abound — but only if you fail to anticipate them. This article outlines the major perpetrators of false results and how to prevent them. For a more general overview of…

17 Oct 2019 PCR&PCR, qPCR and qRT-PCR

CRISPR-Based Activation (CRISPRa) of Genes: A How-To Guide

The purpose of this article is to walk you through the steps needed to overexpress genes using CRISPR/Cas9-based activation (CRISPRa). A broader overview of this topic (including CRISPR-based repression) can be found here. CRISPR/Cas9 is more than a programmable nuclease. When stripped of its nuclease activity, it can activate and repress transcription, alter chromatin structure,…

04 Oct 2019 Genomics and Epigenetics&Sigma-Aldrich® Advanced Genomics
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