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Patrick Miller-Rhodes

"Patrick is equal parts neuroscientist, writer, and extreme sports enthusiast. He is currently a Ph.D. candidate at the University of Rochester Medical Center, where he uses molecular biology techniques to study inflammation and neurodegeneration. To flesh out his scientific and medical writing portfolio, he pursues freelance writing opportunities when he’s not at the bench or off the grid.

Articles by Patrick Miller-Rhodes:

Multiplex CRISPR Gene Editing: What’s It For?

The RNA-guided nature of CRISPR nucleases (e.g., Cas9, Cpf1) makes them highly amenable to multiplex applications, including multi-gene knockout and large-scale genomic modifications. This article — part 1 of a two-part series on multiplex CRISPR gene editing — discusses these applications and outlines practical considerations for multiplex CRISPR gene editing experiments. New to CRISPR gene…

21 Jan 2020 Genomics and Epigenetics

Faster Ligations: PEGing down the Secret

Overnight ligations are inconvenient — especially when they fail. Luckily, there’s a straightforward way to faster DNA ligations. This article highlights the secret ingredient to faster ligation reactions and offers some tips and caveats on its use. For a general overview of DNA ligations, see here and here. Buy a Quick Ligation Kit The most…

27 Nov 2019 DNA / RNA Manipulation and Analysis

How to Prevent False Results in Colony PCR

How to Prevent False Results in Colony PCR Colony PCR saves time and reduces costs by eliminating the need for plasmid purification. However, confounding results abound — but only if you fail to anticipate them. This article outlines the major perpetrators of false results and how to prevent them. For a more general overview of…

17 Oct 2019 PCR&PCR, qPCR and qRT-PCR

CRISPR-Based Activation (CRISPRa) of Genes: A How-To Guide

The purpose of this article is to walk you through the steps needed to overexpress genes using CRISPR/Cas9-based activation (CRISPRa). A broader overview of this topic (including CRISPR-based repression) can be found here. CRISPR/Cas9 is more than a programmable nuclease. When stripped of its nuclease activity, it can activate and repress transcription, alter chromatin structure,…

04 Oct 2019 Genomics and Epigenetics&MilliporeSigma GENM
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