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Natalia Pinello

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Overlap Extension PCR Cloning

Overlap Extension PCR Cloning

By Natalia Pinello | September 12, 2014

If efficient cloning is what you are after, you must give Overlap Extension PCR Cloning a go! This restriction enzyme and T4 DNA ligase-free technique is faster, more reliable and easier to troubleshoot than traditional restriction methods. With only two PCR reactions required, you can insert a DNA fragment into a plasmid without spending time…

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