Natalia is currently a PhD Student in Biochemistry and Molecular Biology at the University of Sydney.
Articles by Natalia Pinello
If efficient cloning is what you are after, you must give Overlap Extension PCR Cloning a go! This restriction enzyme and T4 DNA ligase-free technique is faster, more reliable and easier to troubleshoot than traditional restriction methods. With only two PCR reactions required, you can insert a DNA fragment into a plasmid without spending time…