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Niki Athanasiadou

Niki Athanasiadou received her PhD in Cellular and Molecular biology from the University of Edinburgh. Niki has practical experience and a thorough understanding of big data analytics, from identifying the right question to experimental design and data sampling approaches to applying the appropriate statistical and machine learning method for the task at hand. She is passionate about proper data usage for avoiding biases and the applications of data science in healthcare and public health.

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Articles by Niki Athanasiadou

From ChIP-seq to MeDIP: A Glossary of Different NGS techniques

From ChIP-seq to MeDIP: A Glossary of Different NGS techniques

By Niki Athanasiadou | March 20, 2015

It was not long since the commercialization of NGS (a little more than ten years ago) that scientists went beyond the basics and got creative with the new technology to study much more than just the sequence of DNA. In this article we highlight some of the different NGS technologies and methods available out there.…

A Quick-Fire Guide to Shotgun Sequencing (and Assembly)

A Quick-Fire Guide to Shotgun Sequencing (and Assembly)

By Niki Athanasiadou | March 6, 2015

“Making the simple complicated is commonplace; making the complicated simple, awesomely simple, that’s creativity.” –  Charles Mingus   Next Generation Sequencing (NGS) technology has boomed in recent years, allowing researchers to probe further into the workings of the genome. According to the theory of simplicity, it is the simple principles on its basis that make…

Sequencing the microbiome: re-discovering our invisible allies

Sequencing the microbiome: re-discovering our invisible allies

By Niki Athanasiadou | September 11, 2014

Scientific breakthroughs have profound effects on how we view and treat the world that surrounds us. Especially in the microscopic level, scientific discoveries provide the only understanding we have of Nature. One such case is the story of our relationship with microorganisms. First impressions Since Luis Pasteur’s discoveries on invisible “infectious agents” more than 150…

NGS and TRex: The Science Of Jurassic Park

By Niki Athanasiadou | September 26, 2013

The necessity to see negative scientific results in academic journals has recently drawn some attention. However, for the working experimentalist, it is still unthinkable that he or she would be able to publish meticulous experiments that have painstakingly produced, well, nothing! That is unless Hollywood has made your experiment part of the popular culture. A satisfying…

Solid Phase Reversible Immobilization: How To Get A Bead On The Clean-up Of Your NGS Libraries

By Niki Athanasiadou | June 13, 2013

“Any sufficiently advanced technology is indistinguishable from magic.” – Arthur C. Clarke In the fast-moving field of next generation sequencing, standard practices are evolving rapidly. Today, more and more labs are using Solid Phase Reversible Immobilization (SPRI) beads instead of gel purification in the preparation of libraries for sequencing. A crucial step, not for the…

Celebrating The 60th Anniversary Of The Publication Of DNA Structure: Epigenetics And NGS Pave The Way Forward

By Niki Athanasiadou | April 25, 2013

Today, the 25th of April 2013, marks the 60th anniversary since the groundbreaking publication on DNA structure by James Watson and Francis Crick. In recent years we are witnessing another revolution in biology; epigenetics. And, like figuring out the structure of DNA required the development of X-ray technology, so epigenetics is intimately linked with next generation sequencing.…

Ignore The Ticking Bomb At Your Peril: RNA-Seq Normalization- A Lurking Problem And It’s Solution.

By Niki Athanasiadou | March 14, 2013

You have spent days, if not weeks, at the bench setting up the treatment and control samples for that crucial experiment. You submitted your cDNA library for sequencing and after a few weeks of waiting anxiously you get back a list of differentially expressed genes. Hooray?! Hold on- not quite yet! There is something you…

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