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Niki Athanasiadou

Niki Athanasiadou received her PhD in Cellular and Molecular biology from the University of Edinburgh. Niki has practical experience and a thorough understanding of big data analytics, from identifying the right question to experimental design and data sampling approaches to applying the appropriate statistical and machine learning method for the task at hand. She is passionate about proper data usage for avoiding biases and the applications of data science in healthcare and public health.

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Articles by Niki Athanasiadou

From ChIP-seq to MeDIP: A Glossary of Different NGS techniques

From ChIP-seq to MeDIP: A Glossary of Different NGS techniques

By Niki Athanasiadou | March 20, 2015

It was not long since the commercialization of NGS (a little more than ten years ago) that scientists went beyond the basics and got creative with the new technology to study much more than just the sequence of DNA. In this article we highlight some of the different NGS technologies and methods available out there.…

A Quick-Fire Guide to Shotgun Sequencing (and Assembly)

A Quick-Fire Guide to Shotgun Sequencing (and Assembly)

By Niki Athanasiadou | March 6, 2015

“Making the simple complicated is commonplace; making the complicated simple, awesomely simple, that’s creativity.” –  Charles Mingus   Next Generation Sequencing (NGS) technology has boomed in recent years, allowing researchers to probe further into the workings of the genome. According to the theory of simplicity, it is the simple principles on its basis that make…

Solid Phase Reversible Immobilization: How To Get A Bead On The Clean-up Of Your NGS Libraries

By Niki Athanasiadou | June 13, 2013

“Any sufficiently advanced technology is indistinguishable from magic.” – Arthur C. Clarke In the fast-moving field of next generation sequencing, standard practices are evolving rapidly. Today, more and more labs are using Solid Phase Reversible Immobilization (SPRI) beads instead of gel purification in the preparation of libraries for sequencing. A crucial step, not for the…

Ignore The Ticking Bomb At Your Peril: RNA-Seq Normalization- A Lurking Problem And It’s Solution.

By Niki Athanasiadou | March 14, 2013

You have spent days, if not weeks, at the bench setting up the treatment and control samples for that crucial experiment. You submitted your cDNA library for sequencing and after a few weeks of waiting anxiously you get back a list of differentially expressed genes. Hooray?! Hold on- not quite yet! There is something you…

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