Nat Graham

Nat received his biology degree from Truman State University in 2011 and is currently a PhD candidate at the University of Missouri-Columbia. After gaining an appreciation for the scope of the impending food crisis, he has decided to spend his research time focusing on improving crop modification technologies. His current work focuses on chromosome modifications and the creation of minichromosomes in maize.

Articles by Nat Graham:

An Introduction to Fertilizers in Plant Research

If you have ever had a home garden, you are probably familiar with the fact that adding a little fertilizer to a plant can really do wonders. This can also be the case in a lab greenhouse! The difference is that instead of adding a bit of the “blue stuff,” we try to be a…

14 Mar 2018 Cells and Model Organisms

How to Manage Greenhouse Pests

In my last article I introduced what it takes to work in a greenhouse. While for the most part it is a pretty simple work environment, there is one aspect that warrants a more in-depth discussion: greenhouse pests. It doesn’t matter if the pest is an insect, virus, or bacteria, it can very quickly bring…

26 Sep 2017 Cells and Model Organisms

Tips and Tricks to Get Around Low Plasmid Yields in Agrobacterium tumefaciens

A while back, one of our readers asked for a quick and easy and quick way to extract plasmids from transformed Agrobacterium tumefaciens cells. They pointed out that plasmid copy number is often low in Agrobacterium and that yield can be poor in alkaline base miniprep protocols. The short answer is that there is no…

26 Apr 2017 DNA / RNA Manipulation and Analysis

Earn That Green Thumb! An Introduction to Working in a Greenhouse

If you have worked in a lab before, you probably think you are prepared to work anywhere. You’ve done the safety classes, know how to store the chemicals, even know how to work the chemical shower. Unfortunately, that doesn’t fully prepare you for greenhouse work. Greenhouses are a different kind of greenery-filled animal entirely, and…

12 Mar 2017 Basic Lab Skills & Know-how&Cells and Model Organisms

Cloning Large or Complex DNA Fragments

Sometimes you know a project is going to be a pain before you even start it. For me, that is whenever I need to clone large (> 3 kb) or complex (e.g., a sequence with repeats) DNA fragments. Long and complex DNA fragments are more likely to create challenges during cloning. Such projects require extra care in just about…

21 Feb 2017 DNA / RNA Manipulation and Analysis