Melanie Laederich

Articles by Melanie Laederich:

How to Make a Custom Affinity Medium for Protein Purification

Is your goal to purify a substantial amount of a specific protein? Do you have a quantity of a molecule that binds your protein of interest? If so, generating a custom affinity matrix may be just the trick you need to purify your protein of interest by affinity chromatography. Customizing your affinity chromatography is an…

09 Jul 2016 Protein Expression & Analysis

The 4 Important Steps for Western Blot Quantification

As scientists we love nothing more than quantitative data! Oh ya! But if you don’t quantify your Western blots correctly you’ll find yourself in an unpleasant, unrepeatable and totally meaningless place. And while some scientists are okay dwelling in a meaningless place, I hope you are not. Review these important concepts about how to correctly…

21 Mar 2015 Protein Expression & Analysis

Acid Wash, Autoclave, Flame or Coat? Slide Basics Explained

There are about as many protocols to prepare coverslips as there are ways to make tuna casserole. You can spend from 5 seconds to 2 days, depending on what your lab prefers. But in the end, what’s really needed? I’ve tried many protocols over the years and I’ve questioned some steps. Here I share my…

24 Feb 2015 Microscopy & Imaging

Resolution and Refractive Index: Set-up Your Confocal Wisely!

The most important goal of any confocal microscopy experiment is to maximize photon collection. Forgetting the basics about how photons travel back to your detector is not an excuse for bad data. In this article I will tell you how to increase your resolution by optimizing the refractive indexes in your experiment. By reviewing a…

24 Jan 2015 Microscopy & Imaging

Keeping Your Proteins Happy with Chemical Chaperones

Chemical chaperones are necessary in protein experiments. From buffers to storage solutions, chemical chaperones silently make proteins happy and soluble. Read this article to appreciate the love that chemical chaperones bathe on your proteins and learn when to use them! A chemical chaperone is a molecule that promotes the favorable interaction of protein with water…

18 Jan 2015 Protein Expression & Analysis