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Melanie Laederich

Melanie has a PhD from Oregon Health and Science University School of Medicine. She is a Post Doctoral Fellow at UC San Diego, studying the signal integration and crosstalk between G-protein coupled receptors (GPCRs and G-proteins) and Receptor Tyrosine Kinases (RTKs) in cancer and in diseases of the central nervous system.

Institution : UC San Diego
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Articles by Melanie Laederich

light being defracted by a prism to represent optimizing the refractive index in confocal microscopy

Resolution and Refractive Index: Set-up Your Confocal Wisely!

By Melanie Laederich | February 5, 2021

Get the best out of your time on the microscope by understanding the refractive index of your experiment to optimize and increase your resolution.

How to Make a Custom Affinity Medium for Protein Purification

How to Make a Custom Affinity Medium for Protein Purification

By Melanie Laederich | July 9, 2016

Is your goal to purify a substantial amount of a specific protein? Do you have a quantity of a molecule that binds your protein of interest? If so, generating a custom affinity matrix may be just the trick you need to purify your protein of interest by affinity chromatography. Customizing your affinity chromatography is an…

The 4 Important Steps for Western Blot Quantification

The 4 Important Steps for Western Blot Quantification

By Melanie Laederich | March 21, 2015

As scientists we love nothing more than quantitative data! Oh ya! But if you don’t quantify your Western blots correctly you’ll find yourself in an unpleasant, unrepeatable and totally meaningless place. And while some scientists are okay dwelling in a meaningless place, I hope you are not. Review these important concepts about how to correctly…

Acid Wash, Autoclave, Flame or Coat? Slide Basics Explained

Acid Wash, Autoclave, Flame or Coat? Slide Basics Explained

By Melanie Laederich | February 24, 2015

There are about as many protocols to prepare coverslips as there are ways to make tuna casserole. You can spend from 5 seconds to 2 days, depending on what your lab prefers. But in the end, what’s really needed? I’ve tried many protocols over the years and I’ve questioned some steps. Here I share my…

Keeping Your Proteins Happy with Chemical Chaperones

Keeping Your Proteins Happy with Chemical Chaperones

By Melanie Laederich | January 18, 2015

Chemical chaperones are necessary in protein experiments. From buffers to storage solutions, chemical chaperones silently make proteins happy and soluble. Read this article to appreciate the love that chemical chaperones bathe on your proteins and learn when to use them! A chemical chaperone is a molecule that promotes the favorable interaction of protein with water…

Overview of Protein Turnover using 35S: how to prevent trashy data and uh-oh moments

Overview of Protein Turnover using 35S: how to prevent trashy data and uh-oh moments

By Melanie Laederich | December 16, 2014

The half-life of a protein is an important factor in many molecular biology studies. If your thesis has anything to do with proteins then your graduate advisory committee will ask about half-life, so start planning your 35S experiment today. To help you here is my overview of the major steps of 35S labeling complete with…

The Five X-Factors in Bacterial Protein Production and Purification

The Five X-Factors in Bacterial Protein Production and Purification

By Melanie Laederich | November 4, 2014

Every protein is unique and thus every protein has its own set of production and purification challenges – many of which cannot be predicted. Therefore to successfully produce and purify your favorite protein you need to know and understand these five unpredictable protocol variables (X factors). Tweaking these X factors just might be the difference…

Know Your Western Blot Jargon! A Quick Review.

Know Your Western Blot Jargon! A Quick Review.

By Melanie Laederich | August 19, 2014

It has been said that “Incomprehensible jargon is the hallmark of a profession” (Kingman Brewster, Jr) and while I cannot speak for other professions, as a biologist I am inclined to believe it. So whether you need to cover your qualifying exam bases, want to avoid looking like an idiot to your coworkers, or need…

The Top 10 Western Blotting Mistakes (and Solutions!)

The Top 10 Western Blotting Mistakes (and Solutions!)

By Melanie Laederich | April 1, 2014

As bitter experience has likely taught you, not all Western blots are pretty.  Sadly this is usually due to mistakes on the experimenter’s part. While some of these mistakes are perplexing, others are just plain dumb; but none of them have to happen to you. Read this! 1. Starting with Bad Samples Proper protein extraction…

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