Louise Hughes
Articles by Louise Hughes:
Cryofixation and Chemical Fixation for Electron Microscopy
Specimen preparation is the most important aspect of biological electron microscopy (EM), as it influences everything from the preservation of the sample itself to the kind of information that can be obtained. It is vital to define the questions you are asking of the sample before embarking on an electron microscopy project. This enables you…
Ultramicrotomy for Electron Microscopy
Ultramicrotomy is the process by which a sample is cut into very thin slices or “sections”, usually for imaging by transmission electron microscopy (TEM) or relatively new techniques using scanning electron microscopy (See Array tomography in three dimensional scanning electron microscopy for biology). This technique requires a bit of finesse, and this article will help…
An Introduction to Electron Microscopy for Biologists
Electron microscopy (EM) is a fantastic tool that enables biologists to capture images of their samples at a greater resolution than with a light microscope. There are several types of EM and each of these can provide different information about your sample. The large field of EM is expanding all the time and there are…