Articles by Kimberly Jasmer-McDonald:
So, you’ve designed PCR primers to amplify your sequence of interest, and you’re ready to go. But unless you have a never-ending supply of template, polymerase, and a thermocycler with a gradient function—not to mention a hefty dose of time and patience—you probably don’t want to spend the next week finding the perfect conditions for…
You open the incubator in the morning and to your dismay there are a hundred glorious colonies… on your vector-only control plate. While there are a number of potential causes, I’ll highlight a few of the more likely culprits and their solutions.
As a new postdoc, I found myself in an uncomfortable situation. In my new environment, I am constantly in contact with my mentor and fellow lab members. I must regularly communicate with them and I have routine access to a sounding board for advice and troubleshooting. While I recognize the value of this situation, I initially found it difficult to navigate.