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Kayly Lembke

Articles by Kayly Lembke:

RNA Isolation from Drosophila – Don’t Let the Cuticle Scare You!

Isolating RNA from either Drosophila larvae or adult heads can seem a bit daunting, primarily due to the presence of the cuticle. The cuticle is a protective exoskeleton comprised of insoluble collagens, cuticlins, glycoproteins, and lipids. While it may take some force to remove the cuticle, you can do this easily and without compromising your…

21 May 2018 DNA / RNA Manipulation and Analysis

Simple Tips for a Clean(-ish) Lab Drawer

Picture it: 6:00 pm on a Friday night. You have one or more experiments running. Maybe you’re doing a western blot, or following a staining protocol for an immunohistochemistry experiment, or just labeling tubes. But rather than working on active experiments, you’re helplessly searching through the lab drawer for that one pair of forceps, that…

03 Apr 2018 Basic Lab Skills & Know-how&Organization & Productivity

How to Pour Agar Plates in a Pinch

Every lab has a culture, a vibe of its own. Nowhere does the distinct character of the lab become most apparent than the way in which the lab chooses to pour agar plates. You may have heard or been told to pour plates at some point in your lab career. These “plates” could be called…

30 Jan 2017 Basic Lab Skills & Know-how

The 31 Flavors of Drosophila Electrophysiology Recording Solutions

Lab techniques are often shrouded in a veil of perceived voodoo and superstition – almost everything and anything a person does in lab can have an associated dance, prayer, or ritual to ensure their success. This is especially true for Drosophila electrophysiology. From lab to lab there are different prescriptions for electrode resistance, dissection technique,…

09 Jul 2016 Cells and Model Organisms