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Jeff Mayfield

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Articles by Jeff Mayfield

Optimize Bacterial Protein Expression

Optimize Bacterial Protein Expression by Considering these 4 Variables

By Jeff Mayfield | November 30, 2016

So, you have successfully cloned your gene of interest and are eager to purify buckets of protein. No matter your eventual application—kinetic experiments using a SPR instrument, structural analysis using X-ray crystallography, or any other experiment—you’ll need to express your protein first.  Now, it’s time to put your expression plasmid into E. coli and get…

How to Make Your Own Luck in a Biochemistry Lab

How to Make Your Own Luck in a Biochemistry Lab

By Jeff Mayfield | July 9, 2016

During one of my first ever poster sessions, I was asked how I was able to make a particularly difficult-to-work-with protein soluble. My response was that I was just lucky. The venerable professor chuckled and stated that in science “you make your own luck.” This is a phrase that I have heard quite a bit…

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