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Jeff Mayfield

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Optimize Bacterial Protein Expression

Optimize Bacterial Protein Expression by Considering these 4 Variables

By Jeff Mayfield | November 30, 2016

So, you have successfully cloned your gene of interest and are eager to purify buckets of protein. No matter your eventual application—kinetic experiments using a SPR instrument, structural analysis using X-ray crystallography, or any other experiment—you’ll need to express your protein first.  Now, it’s time to put your expression plasmid into E. coli and get…

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