James Hadfield

James received a Biology degree from the University of East Anglia in 1995, and his PhD in 2014. His career so far has leant towards technology development or implementation. In 1995 he developed a differential PCR test for ErbB2 copy number and over the last 16 years he's worked at; the Norfolk & Norwich Hospital, Royal London on Diabetes genetics, the Cambridge Uni Department of Pathology and the John Innes Centre on Wheat disease resistance gene cloning and arrays. In 2000 he set up an Affy and spotted microarray facility at JIC, he co-founded the UK Affy user group, which is still going strong. Whilst at JIC he also won a Biotech competition, and hopes one-day to start a business although none of his ideas have come to anything yet! In 2006 James moved to set up the genomics facility at the CRUK Cambridge Institute, a department of the University of Cambridge. The lab offers broad spectrum genomic services for scientists at CRI and Illumina next-gen sequencing for CRI, Gurdon, LMB, IMS, Stem Cell, Obs & Gynae and Haematology. His interests today are firmly in next generation sequencing and development of the technology for personalised medicine. He also writes the Core Genomics blog, commenting on the exciting and fast moving world of Genomics. With a focus on next-generation sequencing and microarray technologies, although it is does go off on tangents from time-to-time. Publications by James Hadfield

Articles by James Hadfield:

Where Did It All Go Wrong?! Quality Control For Your NGS Data

You’ve carefully collected your samples, extracted nucleic acids and made your first set of next-generation sequencing libraries. How are you going to know if the data you get back is any good and whether it will be worth the effort in learning how to do the analysis? Who is to blame? Fortunately, there are several…

09 Jul 2016 Genomics & Epigenetics

An Introduction to RNA-seq

RNA sequencing (Wang 2009) is rapidly replacing gene expression microarrays in many labs. RNA-seq lets you quantify, discover and profile RNAs. For this technique, mRNA (and other RNAs) are first converted to cDNA.   The cDNA is then used as the input for a next-generation sequencing library preparation. In this article, I’ll give a brief…

09 Jul 2016 Genomics & Epigenetics

Nanopore Sequencing: An Update

People would have said that a USB sequencer not much bigger than a memory stick which could sequence genomes in 50kb+ read-lengths was impossible “’Star Trek’ technology!” Now, that futuristic technology is here.

09 Jul 2016 Genomics & Epigenetics

Why Is It Important To Run Your NGS Gels Consistently?

This article discusses some of the important things to consider if you are using agarose gel electrophoresis for size-selection of your NGS libraries. Gel electrophoresis is a simple and very commonly used technique in most labs. Careful! It’s a critical step However this simplicity means people can often overlook the fact that there are applications where…

16 Jul 2015 Genomics & Epigenetics

Quantifying Your NGS Libraries

If you want to get the maximum yield and quality from your next-generation sequencing experiment then you are going to need to make sure each of the libraries you produce is carefully quantified ready for pooling and/or loading onto a flow cell. If the quantification goes wrong you’ll get a bad balance of samples within…

20 Feb 2015 Genomics & Epigenetics