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Gaetan Burgio

Gaétan has a PhD in Genetics from the Pasteur Institute, and is currently Group Leader at John Curtin School of Medical Research, Austria.

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Articles by Gaetan Burgio

How to Test the Efficiency of your sgRNA

By Gaetan Burgio | June 6, 2017

To successfully edit your genome of interest, one critical step is to test the sgRNA you have designed. Fortunately there are programs that have been developed such as CRISPRscan for zebrafish, SSC, Sequence Scan for CRISPR, or WU-CRISPR that you can use to predict the efficiency and the suitability of the sgRNA. However, the prediction…

Get Started in Genome Editing with CRISPR

Get Started in Genome Editing with CRISPR

By Gaetan Burgio | July 9, 2016

The development of CRISPR/Cas9 technology has made it relatively straightforward to selectively edit genomes and has revolutionized the way in which we approach biological questions. CRISPR stands for Clustered Regularly Interspaced Short Palindromic Repeats and in simple terms, this technique allows you to direct a nuclease to cut at a specific site in the genome of interest. The…

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