Emily Crow

Emily Crow has a PhD in Life Sciences from Northwestern University. She is currently working as an editor for a scientific publishing company. Her many scientific interests include microbiology, parasitology, and prion diseases.

Articles by Emily Crow:

How To Preserve Your Samples In Western Blotting

When running a quantitative Western blot, it’s crucial that your sample preparation is consistent.  Incomplete protein extraction from one sample will skew your results when you compare it to the protein content of a sample that was extracted more thoroughly.  And after the protein extraction, it’s important to handle the samples in an identical manner…

19 Mar 2012 Protein Expression and Analysis

3 Approaches to Western Blot Transfer

I think that transferring Western blots is one the most enjoyable tasks to do in a lab: it’s quick, it’s messy, and on some gleeful level, it feels like a child’s art project gone wrong.  Of course, it’s also finicky and slippery and prone to tiny pitfalls that can noticeably affect the quality of your…

05 Mar 2012 Protein Expression and Analysis

How Do YOU Make Sure That Your Western Blots are Evenly Loaded?

For Western blot data to be reliable, it is important that you load known amounts of sample into each lane of the gel.  This is of particular importance if you are doing a quantitative blot, where you really need to be able to compare band intensity in each sample.  In this article, we’ll talk about…

27 Feb 2012 Protein Expression and Analysis

Mind Your P’s And Q’s: A Short Primer On Proofreading Polymerases

For applications such as site-directed mutagenesis, it is often recommended that you use a proofreading polymerase (also known as high-fidelity polymerases) to minimize the risk of introducing unintended point mutations.  But what is a proofreading polymerase?  What makes them different from other polymerases?  And when should you use them?  Read on to learn more… What…

11 Jan 2012 PCR, qPCR and qRT-PCR&Qiagen

Are Purified Primers Really Necessary For Site-Directed Mutagenesis?

Most site-directed mutagenesis protocols strongly recommend that you use only PAGE- or HPLC-purified primers to mutate plasmid templates.  Using purified primers is supposed to minimize the introduction of unintended mutations, thus drastically improving the probability of generating your desired mutant.  However, specially purified primers can be extremely expensive, and take longer to synthesize than standard…

06 Jan 2012 DNA / RNA Manipulation and Analysis
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