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David Galbraith

David Galbraith obtained undergraduate and graduate degrees in Biochemistry from the University of Cambridge, and postdoctoral training as a NATO Fellow at Stanford University. His first academic appointment was at the University of Nebraska Lincoln, and he became Professor of Plant Sciences at the University of Arizona in 1989. His research has focused on the development of instrumentation and methods for the analysis of biological cells, organs, and systems. He pioneered the use of flow cytometry and sorting in plants, developing widely-used methods for the analysis of genome size and cell cycle status, and for the production of somatic hybrids. He also developed methods for the analysis of gene expression within specific cell types, using markers first based on β-glucuronidase, and subsequently on Fluorescent Proteins. Current interests include applications of highly parallel platforms for transcript profiling of minimal sample sizes, and for analysis of genetic and epigenetic mechanisms that regulate gene expression during normal development and in diseased states. He has published more than 160 scholarly research articles, holds several patents, and was elected a Fellow of the American Association for Advancement of Science in 2002.

Articles by David Galbraith:

Sorting Large Cells and Materials by Flow Cytometry

Flow cytometers and cell sorters were designed with blood cells in mind. This means that commercial cell sorters are optimized for sorting cells typically smaller than about 20 µm in diameter. However, it turns out that many cell types, including those of mammals, are larger than 20 µm. So what are your options if you…

sorting large cells
09 Jul 2016 Flow Cytometry

Publish and Perish: Post-Publication Review and Why YOU Should Get Involved

“13 Photoshop Fails You Won’t Believe Really Happened!”  A familiar tag-line on the internet, and you yet again waste a few minutes as you click through a series of impossible images of the human form. The word Photoshop, as for Xerox, has lost its Trademark status through the compliment of being incorporated into everyday English:…

01 Jun 2015 Writing, Publishing and Presenting

Crap in, crap out: Flushing Out The Problems in Your Flow Cytometry Data

“What Have You Done To My Cells??!!!” This cry of pain from researchers, frequently aimed at core facility operators, is heard after receiving incomprehensible data for an invaluable tube of cells. Equally baffling to the trained user of flow cytometric instrumentation is when data emerges that is either unreliable or inconsistent with the known properties…

09 Sep 2014 Adaptive Biotechnologies&Flow Cytometry

Sorting Single Cells – What Do You Need to Consider?

Flow cytometer and cell sorter manufacturers have invested considerable resources to design instruments that are the “fastest in the ‘hood” either in terms of cells analyzed per second, or in total throughput. The general idea is the faster you can go, the quicker you can identify rare cells, and produce sorted populations containing large numbers…

08 Jul 2014 Adaptive Biotechnologies&Flow Cytometry
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