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Chelsey Kline

Articles by Chelsey Kline:

Crash Course in Fourier Transform Infrared Spectroscopy

Fourier transform infrared spectroscopy (FTIR) is an extremely sensitive technique for measuring the absorption and intensity of electromagnetic radiation in the infrared region of the spectrum of either a solid, liquid or gas sample. You can use FTIR to: quantify unknown compounds identify unknown compounds study the detailed structured coordination of compounds How Does Fourier…

18 Jul 2018 Analytical Chemistry and Chromatography Techniques&Protein Expression and Analysis

How to Prepare Biological Metallo-Proteins

The first thing one might notice when working with metallo-proteins is that they offer unique, colorful reactions.  These colorful reactions are based not only on the metal, but the ligand, or coordinating molecules.  Approximately 80% of proteins contain inorganic cofactors like iron (Fe) and copper (Cu) metals necessary to catalyze a reaction.  Understanding how these…

12 Sep 2017 Protein Expression and Analysis

The Nitty Gritty’s of Cell Culture Techniques

Mammalian cell culture techniques are not something you learn from a book, per se. And because of this, it is important to be properly trained, especially in sterile techniques. It is important to keep your cell lines from contamination and just as important to keep yourself safe. Nevertheless, people tend to do things a little…

07 Sep 2017 Cells and Model Organisms

Labeling For Life – Get a Good Self-Tracking Labeling System

When you work in a laboratory, preparing samples sets for many different experiments is a large part of the job. Keeping track of your samples can be tedious or even challenging if you don’t already have a good system in place. However, getting this right is a critical part of the experimental process. In this…

31 Aug 2017 Basic Lab Skills and Know-how

The Night Shift: Experiences at a National Synchrotron

Pulling an over-nighter is a common theme for many of my college friends. I, however, like my sleep—particularly between midnight and 7 am. Therefore, I tend not to stay up late to study and avoided jobs that required me to work a night shift. Then, I went to grad school and had to put in…

10 Jul 2017 Survive and Thrive