Cindy Duarte Castelão

Cindy received a Biomedical degree in 2011, and a MsC in Molecular Biology and Genetics in 2011. Her career so far has been in the Molecular Genetics field, working closely with deep-sequencing techniques, and how these techniques can help to unveil the secrets of the genome and epigenome. Join me in uncovering the mystery. Publications by Cindy Duarte Castelão

Articles by Cindy Duarte Castelão:

How to Survive a Difficult PCR

I am sure many of you have been there. Everything is going smoothly, and your project seems to be working out perfectly. And then there is this one PCR. For some reason, it just won’t work. It is a black dot on your record. Even though I have a scientific mind, I have to be…

12 Oct 2017 PCR, qPCR and qRT-PCR

Next Gen Sequencing Challenges

Next generation sequencing opened the doors to our genome. It gives massive amounts of information in a week – whereas Sanger sequencing takes thrice as long, and causes lab lesions due to the abusive use of pipettes. Indeed, with minimal hands-on procedures we obtain a lot of data. But nothing in Science is ever easy.…

05 Sep 2017 Genomics & Epigenetics

Maxam-Gilbert Sequencing: What Was It, and Why It Isn’t Anymore

In the mid-1970s, two methods were developed for directly sequencing DNA: the Maxam-Gilbert sequencing (or chemical sequencing) method and the Sanger chain-termination method. Indeed, in 1980, both Walter Gilbert and Frederick Sanger were awarded The Nobel Prize in Chemistry for “their contributions concerning the determination of base sequences in nucleic acids”. Actually, each got a…

26 Jul 2017 Genomics & Epigenetics

An Introduction to Nanopore Sequencing

DNA sequencing is the most powerful method to reveal genetic variations at the molecular level, leading to a better understanding of our body in physiological settings, and pathological conditions. It is the beginning of the long road towards better diagnostics and personalized medicine. Even though there have been great advances in DNA sequencing technologies there…

18 Jul 2017 Genomics & Epigenetics

Oligo Purification Methods: How, Why and for What?

Who amongst us hasn’t had the need for oligonucleotides in an experiment? It is a cornerstone in many procedures and techniques. Depending on the goal, it can be very hard to design just the right oligo for your experiment.  Oligos must have the right length; the right amount of C-G, T-A; they can’t form secondary…

18 Jul 2017 PCR, qPCR and qRT-PCR