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Since the early 80s, innovators at Biosearch have refined the chemistry of custom oligonucleotide synthesis to accelerate the discovery and application of genomic information. Our unmatched selection of oligo modifications is complemented by our demonstrated expertise in the design and manufacture of fluorogenic probes and primers.

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Challenges of Autofluorescence in Neuroscience

Challenges of Autofluorescence in Neuroscience

By LGC Biosearch Technologies | November 30, 2016

If you have ever imaged biological samples, you have likely encountered autofluorescence. That pesky background coloration you see under the microscope, which can make it difficult to distinguish your actual signal from the noise.1 When you are trying to look for something as delicate as RNA, you don’t want to be hunting for your signal…

Detecting Signal in qPCR: From DNA Binding Dyes to BHQ Probes

Detecting Signal in qPCR: From DNA Binding Dyes to BHQ Probes

By LGC Biosearch Technologies | June 2, 2015

A Brief History of Detecting Amplicons The Old Days of Ethidium Bromide In the early 1990s, quantitative (q) PCR was in its infancy, and despite PCR itself already being around for 10 years, there were no easy ways of precisely quantifying the amount of DNA that was amplified in a PCR reaction. In those days PCR…

Modify Your Oligos, Modify Your Experiments

Modify Your Oligos, Modify Your Experiments

By LGC Biosearch Technologies | March 4, 2015

If you’ve ever performed PCR, you’re probably already very familiar with DNA oligonucleotides (or oligos). But did you know that these molecules can do so much more than just act as simple primers? You can add a wide range of modifications to your oligos, which may change the stability, binding, solubility and even visibility, to…

How RNA-FISH Can Complement qPCR, and Vice Versa

How RNA-FISH Can Complement qPCR, and Vice Versa

By LGC Biosearch Technologies | January 16, 2014

In studies of RNA abundance and gene expression, no one technique can answer all of the questions that need to be asked. So it is necessary to use a variety of experimental methods in concert. Two RNA detection and measurement techniques that complement each other well for this purpose are RNA Fluorescence in situ hybridization…

A primer on Probe-based SNP genotyping

A primer on Probe-based SNP genotyping

By LGC Biosearch Technologies | December 13, 2013

Single Nucleotide polymorphisms (SNPs), colloquially pronounced ‘snips’, are the most common type of genetic variation in people. By definition, a SNP represents a single nucleotide variation at a specific location in the genome that is found in more than 1% in the population. For example, a SNP can replace the nucleotide cytosine (C) with an…

A Quick Tour Around Probe-based Multiplexing qPCR

A Quick Tour Around Probe-based Multiplexing qPCR

By LGC Biosearch Technologies | November 29, 2013

PCR has become the tool of choice for molecular diagnostics and is now a staple platform in any laboratory setting. The versatility of this method has led to a myriad of spin-off techniques, including probe-based quantitative PCR (qPCR). This method effectively combines PCR amplification and detection into a single step to measure the specific amount…

PCR is 30! Here's How it has Rocked the World

PCR is 30! Here’s How it has Rocked the World

By LGC Biosearch Technologies | November 15, 2013

This year marks an important birthday for molecular biologists: the polymerase chain reaction (PCR) turns 30!  This is my eulogy to celebrate life so far for the technique that has rocked the world. PCR has become such an integral part of the bioscientist’s toolbox that new generations of researchers probably cannot imagine life without it. It…

How to detect long non-coding RNA (lncRNA)

How to detect long non-coding RNA (lncRNA)

By LGC Biosearch Technologies | September 29, 2013

According to the central dogma of molecular biology, DNA is transcribed into RNA, that is translated to proteins. Inconveniently, the vast majority of the genome contains sequences that do not actually code for proteins. So, this non-coding RNA (ncRNA) was dismissed as non-functional junk, letting researchers tick the box on their to-do lists and head off…

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