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Aunt Yersinia

Aunt Yersinia is your microbology Agony Aunt at Bitesize Bio. Come to her with all of the questions you were too afraid to ask, and she will keep you right.

Articles by Aunt Yersinia:

How To Ask For Reagents

Greetings, I’m a research student and I’m having a lot of trouble in finding the right antibodies for my study, because I need both monoclonal and polyclonal. And I need to start addressing some other international scientists. The problem is that I don’t know how to address the situation. If they indeed have antibodies that…

09 Jul 2016 Aunt Yersinia

Am I Damaging My E. coli by Spinning at High Speeds?

Dear Aunt Yersinia, A very annoying postdoc in our group keeps telling me off for spinning E.coli at 13K in a tabletop centrifuge. The postdoc claims that high speed damages cytoskeleton and this will reduce my transformation frequency. But I don’t believe her as the cells are cushioned by water during centrifugation. Can you tell…

18 Mar 2015 Aunt Yersinia

Help: My Gal Promoter Doesn’t Work

Dear Aunt Yersinia, I work with a yeast vector where my gene is under a Gal promoter. My boss told me to grow yeast in medium with sucrose, and then add galactose to induce the promoter. I did it, but my protein is not induced; the Western blot is empty. I sequenced a part of…

18 Aug 2014 Aunt Yersinia

To Pour or to Spread, This is the Question

Dear Aunt Yersinia, What are advantages and disadvantages of pour and spread plates? Antonio   It is refreshing to see such wisdom – knowing that there are at least two methods of pouring plates – in a young man. While all molecular biologists know or have at least seen a ”spread plate” aka Petri dish…

09 Jul 2014 Aunt Yersinia

Microbiology 101 by Aunt Yersinia

We are pleased to announce that the famous maid of microbiology, dear old Aunt Yersinia, has agreed to start writing a microbiology and molecular biology advice column for Bitesize Bio. She will be free to answer your most pressing questions sent to:  [email protected] By way of introduction, Aunt Yersinia is bestowing 8 spores of knowledge garnered…

21 May 2014 Cells and Model Organisms
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