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Ashleigh Miller

Ashleigh gained a Bachelor of Science from UC San Diego.

Discover more about Ashleigh on their professional profiles

Articles by Ashleigh Miller

Protein Lysate TLC: Pro-Tips to Keep Your Protein Extracts in (Experimentally) Perfect Shape.

Protein Lysate TLC: Pro-Tips to Keep Your Protein Extracts in (Experimentally) Perfect Shape.

By Ashleigh Miller | March 4, 2015

Do you need your protein in its native form, intact, with full functionality? Do you need to isolate organelles and nuclear fractions from the cytoplasm? Or do you need a slurry of everything in your cell or tissue? Whatever your experiment, you can maximize the amount of functional, detectable or active proteins by handling your…

The Nature of Denaturing (Protein Gels, that is!)

The Nature of Denaturing (Protein Gels, that is!)

By Ashleigh Miller | November 18, 2014

You’ve nurtured your cells for weeks, perfected your experimental conditions, and nailed down all the controls. You’ve harvested your cells and gently lysed them, now you’re ready to look at the proteins. What’s one of the most common next step in protein analysis? A denaturing gel or SDS-Polyacrylamide Gel Electrophoresis! SDS-Polyacrylamide Gel Electrophoresis, or SDS-PAGE…

Go Fishing for Your Favorite Protein with Immunoprecipitation!

Go Fishing for Your Favorite Protein with Immunoprecipitation!

By Ashleigh Miller | July 15, 2014

You have your favorite protein in mind and are ready to set up some exciting experiments to show what it does and how it does it, when it is active, what other proteins it modifies, and how it affects your cells. There is one slight problem. You need to fish your favorite protein out from the…

You did a Co-IP…now what?

By Ashleigh Miller | April 15, 2014

You spent the last few weeks tweaking your Co-immunoprecipitation conditions, testing different antibody/bead combinations, and sampling a panaply of solutions and FINALLY! You have your Co-immunoprecipitation (Co-IP) elution… Now what? Well, you have a few choices. It really all depends on what you need know about the proteins in your elution. Do you need to identify…

Find Out if Your Proteins Do a Slow Dance Together in the Dark

Find Out if Your Proteins Do a Slow Dance Together in the Dark

By Ashleigh Miller | March 18, 2014

Do you wonder if your favorite protein is entwined in a delicate dance with another protein? Do you wish that you could shine a spotlight on your protein to determine its dance partner? Well good news! While there is no such spotlight, you CAN use co-immunoprecipitation (Co-IP) to find your protein’s dance partner. You can…

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