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Akshata Naik

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stem-loop real-time PCR

From Revolution to Evolution: Stem-loop Real-time PCR

By Akshata Naik | September 27, 2016

Kary Mullis invented polymerase chain reaction (PCR) in 1985 creating a revolution in molecular biology techniques. But it hasn’t stopped there. PCR has greatly evolved over the years. Today, we stand at a point, where we can clone micro RNAs (miRNAs) in real time! Due to miRNA size (about 18-21 nucleotides long) and varied expression levels,…

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