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Amanda Hurley

Amanda Hurley is a postdoctoral associate at the Wisconsin Institute for Discovery applying traditional genetics to bacterial communities. She received a doctorate in Molecular Biology from Princeton University. Amanda grew up on a sheep farm in New Hampshire with two veterinarian parents so her interest in the life sciences was inevitable. Her research focuses on the communication and interactions of bacteria. Communication between humans also interests her. Specifically, advocating for science and the translation of primary scientific research into relatable stories.

Articles by Amanda Hurley:

How it Works: Storage Phosphor Screen

Radioactivity is still the most sensitive detection mechanism for many macromolecules and enzymatic activities. In graduate school, I performed countless radioactive kinase assays, watching the radioactive gamma 32P of ATP get transferred to my autophosphorylating receptor of interest, and then separating my protein from free hot ATP on a gel. The gel is dried, covered…

Zagreb Technical Museum
20 Feb 2019 Protein Expression and Analysis

Old Reliable: Two-Step Allelic Exchange

Manipulating the genes of organisms is crucial for studying their functions. In times before genetic engineering, scientists would shoot bacteria with X-rays or expose them to destructive chemicals until spontaneous mutations would arise. Fortunately, current methods are more sophisticated and less torturous. Researchers now use more directed techniques to introduce mutations. There are several ways…

Old Reliable: Two-Step Allelic Exchange
17 Jul 2018 DNA / RNA Manipulation and Analysis

What the Heck Is “Training Potential,” Anyway?

As a newly-minted PhD, I began my postdoc with wild fellowship dreams. I set a schedule, applying to 1-2 fellowships a month. Research experience and broader impacts were a breeze. Research strategy and specific aims, with help from my new PI, solidified quickly. For weeks, however, my “training potential” document remained empty. At first, I…

20 Mar 2018 Getting Funded

Quantifying Individual Proteins Per Bacterial Cell

I’m a simple molecular biologist. It’s awesome how computational biologists use math to reduce and rebuild biological phenomenon. In my own way, I also like to reduce my observations to numbers. As a budding biochemist, I need to assemble and quantify the players in my pathway to truly understand it. In particular, I am interested…

proteins per cell
19 Jan 2018 Protein Expression and Analysis

Recognizing Anxiety in Graduate School

Graduate school can seem like a constant battle between which of your friends works the hardest and which is the most miserable. However, if being miserable doesn’t interest you, I have some experience recognizing anxiety and seeking help. Depression and anxiety are conditions closely associated with getting a PhD. Experiencing these conditions can be isolating,…

03 Jul 2017 Survive and Thrive&Personal Development

How to Give an Incredible Scientific Talk

Committee meeting approaching? 10 minute department seminar? Lab meeting? Fear not! My adviser has insulted my presentations so regularly, that I’ve finally learned some things. Hopefully, I can head your adviser off at the pass, and give you some tips on crafting an incredible talk. What’s the Occasion? Who’s Your Audience? This is arguably the most important…

06 Apr 2017 Writing, Publishing and Presenting
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