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Amanda Hurley

Amanda obtained a PhD in Molecular Biology from Princeton University. She is currently a postdoctoral associate at the Wisconsin Institute for Discovery.

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Articles by Amanda Hurley

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How it Works: Storage Phosphor Screen

By Amanda Hurley | February 20, 2019

Radioactivity is still the most sensitive detection mechanism for many macromolecules and enzymatic activities. In graduate school, I performed countless radioactive kinase assays, watching the radioactive gamma 32P of ATP get transferred to my autophosphorylating receptor of interest, and then separating my protein from free hot ATP on a gel. The gel is dried, covered…

Old Reliable: Two-Step Allelic Exchange

Old Reliable: Two-Step Allelic Exchange

By Amanda Hurley | July 17, 2018

Manipulating the genes of organisms is crucial for studying their functions. In times before genetic engineering, scientists would shoot bacteria with X-rays or expose them to destructive chemicals until spontaneous mutations would arise. Fortunately, current methods are more sophisticated and less torturous. Researchers now use more directed techniques to introduce mutations. There are several ways…

What the Heck Is "Training Potential," Anyway?

What the Heck Is “Training Potential,” Anyway?

By Amanda Hurley | March 20, 2018

As a newly-minted PhD, I began my postdoc with wild fellowship dreams. I set a schedule, applying to 1-2 fellowships a month. Research experience and broader impacts were a breeze. Research strategy and specific aims, with help from my new PI, solidified quickly. For weeks, however, my “training potential” document remained empty. At first, I…

proteins per cell

Quantifying Individual Proteins Per Bacterial Cell

By Amanda Hurley | January 19, 2018

I’m a simple molecular biologist. It’s awesome how computational biologists use math to reduce and rebuild biological phenomenon. In my own way, I also like to reduce my observations to numbers. As a budding biochemist, I need to assemble and quantify the players in my pathway to truly understand it. In particular, I am interested…


How to Give an Incredible Scientific Talk

By Amanda Hurley | April 6, 2017

Committee meeting approaching? 10 minute department seminar? Lab meeting? Fear not! My adviser has insulted my presentations so regularly, that I’ve finally learned some things. Hopefully, I can head your adviser off at the pass, and give you some tips on crafting an incredible talk. What’s the Occasion? Who’s Your Audience? This is arguably the most important…

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