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Alex Reis

Alex gained a PhD in Embryology, Agriculture and Animal Science from University of Aberdeen.

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Articles by Alex Reis

Should PCR cloning be a part of your molecular cloning toolkit?

Should PCR cloning be a part of your molecular cloning toolkit?

By Alex Reis | July 2, 2014

While the classic approach to molecular cloning – using restriction enzymes to excise a DNA fragment of interest – is as useful as ever, new techniques that make cloning faster, easier and more versatile are available. As a smart molecular biologist, you should be examining each of them to see whether or not adding them…

10 ways to improve blunt-end ligations

10 ways to improve blunt-end ligations

By Alex Reis | January 20, 2014

Blunt-end cloning involves the ligation of DNA fragments – usually between a plasmid vector and an insert – whose terminal ends are not “sticky”. Performing these ligations is notoriously difficult, particularly with large DNA fragments. But it is possible. And in this article I’ll give you some tips that I hope will increase your chances…

Choosing the right plasmid vector: A Guide for beginners

Choosing the right plasmid vector: A Guide for beginners

By Alex Reis | January 13, 2014

If you’re picking up a list of available plasmid vectors for the first time, it can be mind-blowing to decide on the best one for your experiment. What cloning sites do you need? What restriction enzyme sites? What insert size is possible? Let me help you with some pointers about what factors to look out…

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