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Adam Idoine

Adam is a postdoc at the Carnegie Institution, Stanford, where he works on developing molecular biology tools to study various non-model-organism algae. In particular, he’s an expert in transformation, genome editing, transcriptomics and organelle biology. He’s also really enthusiastic about science outreach and communication. He’s currently open to opportunities in the biotech/startup world. In his spare time, he enjoys indoor rock climbing, meet interesting people, and spend time outdoors.

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Articles by Adam Idoine


The qRT-rtPCR Control You Should Be Doing, But Probably Aren’t

By Adam Idoine | December 7, 2017

Every man, woman, and dog is doing quantitative real time reverse transcriptase PCR (qRT-rtPCR) these days. It’s a great method to measure your favorite transcript’s expression levels. One of the big plusses (like the Swiss flag!) of quantitative PCR in general is its high sensitivity. In principle, it can detect and quantify one molecule of…

ribonucleoprotein transformation

Why You Should Use Cas9 Ribonucleoprotein Transformation for CRISPR Genome Editing

By Adam Idoine | October 25, 2017

Imagine directly creating a mutation at (almost) any site in your target genome instead of screening thousands or millions of random mutants! The CRISPR/Cas9 system does just that. In its traditional form, this forward genetics approach takes 7 steps from start to mutated genome. However, there is a way to obtain your designer genome in…

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