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Alexandra Cuaycal

Alexandra gained a PhD in Microbiology and Cell Science from the University of Florida.

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Articles by Alexandra Cuaycal


Bacterial Transformation Troubleshooting for Beginners

By Alexandra Cuaycal | October 30, 2017

The first time I did a transformation was when I worked with site directed mutagenesis. I cloned a protein sequence into the p15TVL vector, created my mutants (but that’s another story), and was finally ready for the next step: transformation and expression of my desired protein. Little did I know that my enthusiasm would fall…

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