Join Us
Sign up for our feature-packed newsletter today to ensure you get the latest expert help and advice to level up your lab work.
Search below to delve into the Bitesize Bio archive. Here, you’ll find over two decades of the best articles, live events, podcasts, and resources, created by real experts and passionate mentors, to help you improve as a bioscientist. Whether you’re looking to learn something new or dig deep into a topic, you’ll find trustworthy, human-crafted content that’s ready to inspire and guide you.
…input sample and the amounts of miRNAs measured, introducing non-linearity in the assay. 3. Next-Generation Sequencing Next-Generation Sequencing (NGS) is fast gaining popularity in biological and clinical laboratories and offers…
…characterize how proteins regulate gene expression by interacting with DNA. ChIP-seq is a major step forward from microarray-based experiments, allowing much higher-throughput interrogations with standard NGS instruments. The process involves…
…In NGS Practically speaking, barcodes in NGS are strings of nucleotides that act as unique identifiers.1 To date, the barcoding strategy is mainly used to tag populations of molecules. As…
…sequencing (NGS), phenotypic assessment, and measuring mRNA and protein levels for your targeted gene. These methods differ in their sensitivity, scalability, resolution, and cost. [1] For example, although NGS offers…
Scientists today depend heavily on many molecular biology techniques to perform their research. For example, with the advent of next generation sequencing (NGS): scientists are able to look at…
The advent of Next Gen Sequencing (NGS) has been truly amazing. One of the marvels that is often overlooked is how advances in DNA extraction technology have helped streamline NGS…
…cellular analytes (such as nucleic acids, proteins, and metabolites) using AFA consistently offers high yield and quality, thereby making this technology well-suited for many workflows, from Next Generation Sequencing (NGS)…
…AWS to Google Cloud) can be complicated. Bridging the Gap: Why Biologists Need the Cloud The Explosion of Biological Data With the rise of next-generation sequencing (NGS), digital microscopy, and…
…– High-throughput flow-cytometry and NGS-based genetic characterization experiments are well-established for cellular platforms, which conveniently package genetic instructions together with their output. Tens of thousands of genetic variants can be…
…all scenarios. Because they require so much hands-on time, these gels make sense for labs that seldom prepare NGS libraries, where sizing is only needed once in a while. They’re…
…Now, as PCR, DNA microarrays, and NGS dominate the DNA analysis scene, about the same number of Southerns are cited as in the 1980s. From chopping to blotting—the birth of…
…identify polyadenylation sites precisely. 1 However, there are some great NGS-based techniques developed specially for polyadenylation site identification, using diverse approaches. The goal of these techniques is to enrich the…
A revolution in 2005 The start of the NGS revolution was clearly marked in 2005 by the publication of the complete genome sequences of two bacterium (Mycoplasma genitalium and Streptococcus…
…variation: high-throughput (HT) SuperSAGE where next generation sequencing (NGS) is employed to analyze up to millions of tags at once! With the introduction of bench-top NGS, HT SuperSAGE is helping…
…the analysis of NGS data. For sure, RNA-seq is a relatively new technology, and it takes some time to adapt pre-existing methods to it. A big breakthrough towards this direction…
…decide which language you’d like to learn. To find out more about how to get started, please check out my upcoming article, ‘Top resources for learning an NGS programming language.’…
…these applications are: Rare allele detection Copy number variation Gene expression for <2-fold differences Quantification of NGS libraries Detecting low-abundance RNA Pathogen detection Viral load detection The Generalist or the…
…a type of Next Generation Sequencing (NGS) that provides an overview of the transcriptome, which includes mRNA as well as: alternative gene spliced transcripts post-transcriptional modifications gene fusions mutations SNPs…
…NGS library quantification, increased sensitivity for detecting target in limited clinical samples and improved multiplexing capability. Here’s how dPCR works The initial dPCR reaction is assembled using the same reaction…
…sequencing (NGS) to determine many interaction partners within a large, pre-determined genomic region.3 This type of experiment is most useful when looking to characterize the overall architecture of a potential regulatory…
…Reading An Easy Way to Start Using R in Your Research – Introduction Online Schools for Learning How to Play in the R Coding Arena Getting Started with NGS Bioinformatics…
…explosive amounts of NGS data. It’s packed with closely set patches in shades of colors, pomping the gene expression data of multifarious high-throughput tryouts. In this article, I will help…
…rapidly decreasing cost of Next Generation Sequencing (NGS) are allowing us to obtain vast amounts of useful genomic information. Ironically, sequencing represents both the end of your molecular biology journey…
…Pooled screens will require the detection of a phenotype that allows for enrichment, such as cell proliferation or activation of a reporter-based assay. [2] 5. NGS and Deconvolution (For Pooled…
…age of shrinking antimicrobial therapies. Curiouser and Curiouser Thus, with a little help from NGS, molecular biologists are finally treading into the realm of higher-order chromosome structure. Their ultimate findings…
…favorite technique to look for the mutation. The most widely used tools here are: Next-Generation Sequencing (NGS) – although this may be an expensive choice, it is very useful if…
…NGS manufacturers sell panels that contain sequences of known cancer genes. For example, Ion Torrent (now part of ThermoFisher Scientific) has a “hot spot” panel that contains 207 primer pairs…
…In addition, if your forays into data analysis have convinced you that you need to learn to code, check out our article on resources for leaning an NGS programming language….
Reliable concentration and purity measurements of DNA, RNA, and protein samples form the foundation of successful cloning, qPCR, NGS library prep, western blots, and countless other molecular biology workflows. However,…
…on your detection method, you can generally detect from as little as a few ngs (50-100 ng if using ethidium bromide) to a microgram. Although you can load more, it…
…example, suppose you conduct clinical-grade NGS testing. In this case, you probably already possess subject matter expertise in clinical genomics and have a good foundation for any role in biocurating…
…and Nanodrop measurements. Nanopore library quality mostly depends on the individual researcher’s technical skills and pipetting technique. In this article, we discuss some pointers to perform a successful NGS library…
…transcriptome, ensuring high-value NGS reads with over 90% rRNA depletion across various organisms. Why Choose Zymo-Seq RiboFree®? Unparalleled Compatibility. Suitable for any organism, including challenging samples like FFPE tissues. Efficiency….
…optics-based technology found in Illumina’s sequencers. In a previous article by James Hadfield, we were briefly introduced to Ion Torrent along with the optical based systems of NGS. Optics-based sequencers…
…other NGS technology has fought against the ‘Big Three’ (454, Illumina, Life Technology). Complete Genomics developed a novel sequencing system using DNA nanoballs and unchained sequencing by ligation (Lee et…
Anyone who is involved in DNA sequencing in one form or fashion knows there are multiple ways to skin a cat: Sanger-based, next generation (NGS), and of course the new…
Technique, tips, ergonomics, and ISO guidance all in one handbook
