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What is a DOI and Why Should You Care?

…their preferred method. If you are using them in your thesis or presentations (poster or digital) speak to your supervisor or institution for their preferred layout and if they have…

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How to Make Sure Your NGS Results are Robust

…don’t, ask questions until you are satisfied with your knowledge. Before publishing your findings, discuss them with colleagues who have expertise in the area. Conferences and presentations are also a…

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The Establishment of the Nobel Prize

…each in Physics, Chemistry, Physiology or Medicine, Literature, and Peace). What’s interesting is that he never picked any Nobel Prize winners, he never went to a prize-giving ceremony, and he…

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Struggles of a Life Scientist

…to the plate reader! To prevent giving the evening cleaning crew a heart attack, keep the sleeping cot at home. Solution: To really up your game, check out the latest…

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The Art of PCR Primer Design

…nucleotides upstream of restriction sites.  This will increase the efficiency of the restriction reaction (by giving the enzyme something to “hold on to”).  Since the extra bases do not anneal…

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Gene Synthesis: Cloning of The Future?

…some clone it in E. coli vector, giving you an unlimited supply of self-correcting template. Having chosen a company it is time to design your sequence. This is the time…

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How to Refold 653 Insoluble Proteins

…yourself a lot of time. And if you don’t, once you develop your protocol, uploading it to the database will save someone else the time in future – giving you…

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The History of PCR

…time-consuming, exhausting, and tedious work. Imagine troubleshooting the optimal temperature for a primer to bind? Rather than popping your plate into a PCR cycler and giving different annealing temperatures to…

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How to: Keep your data organized

…details are lost, it is important to write it up as experiments are performed. Giving each lab book a unique number, numbering the pages, and keeping an index of experiments…

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How Does Peer Review in Science Work? 

…scientists are not paid for peer review work. Reviewing manuscripts for journals is a pro bono job, a service that researchers have been giving for free for centuries. While some…

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