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Microscopy & Imaging

How to See the Cell Cycle Through Your Microscope

Even in the most basic applications, fluorescence microscopy can be a very powerful technique. Simply put, the ability to actually see the biology you are interested in cannot be matched in directness. Often, the aim of fluorescence microscopy is to observe the effect of an experimental manipulation. Ultimately, you would like to know that the…

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The Key to Unlocking DNA from FFPE Tissues

Formalin fixed paraffin embedded (FFPE) tissues are valuable samples that typically come from human specimens collected for examination of the histology of biopsies for the detection of cancer. But each sample contains much more information just waiting to be unlocked. Despite the tiny sample size, DNA can be extracted from the tissue sections and used…

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Immunoscience or Immunoalchemy?

First of all let me say the technique of labeling tissues (immunohistochemistry, IHC), and cells (immunocytochemistry, ICC) is indeed immunoscience NOT alchemy, though at times it may certainly seem like alchemy! But to scientists inexperienced in this technique, who typically see the results of IHC/ICC experiments in the form of pretty pictures, it can certainly…

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An Introduction to Electron Microscopy for Biologists

Electron microscopy (EM) is a fantastic tool that enables biologists to capture images of their samples at a greater resolution than with a light microscope. There are several types of EM and each of these can provide different information about your sample. The large field of EM is expanding all the time and there are…

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Go For Gram! Staining Bacteria for Light Microscopy

The Gram stain is another commonly used special stain in the histology lab. Why use a Gram stain? The Gram stain is a type of differential staining technique which represents an important initial step in the characterization and classification of bacteria using a light microscope. It is named after a Danish scientist, Hans Christian Gram,…

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2015 Staff Picks: Microscopy and Imaging

After looking at pictures of friends and family on holiday cards, head on over to check out some more pictures in the Microscopy and Imaging channel. These are just some of the wonderful articles that we published this year on Bitesize Bio. Jen Redig, Managing Editor Brightness and Contrast in Microscopy Imaging By Alexandros Lavdas…

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How to Make Lipid Bilayers

Supported lipid bilayers are a very useful tool in many fields of cell and membrane biology. But how easy is it to make them? Bilayers can be made quite reproducibly, once you have found a reliable protocol! However, it can take some time to optimize your technique, so to increase your chances, make sure you…

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Illustrated Optical Fiber Glossary (A – E)

Sometimes the biggest hurdle to mastering a new topic is mastering the vocabulary. To help you master optical fibers I put together this illustrated glossary of Optical Fiber Vocabulary. Read this along with my first article “Introduction to Optical Fibers” and you will be well on your way to mastering optical fibers. Acceptance Angle The…

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