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Analytical Chemistry and Chromatography Techniques

Carrying You Through Gas Chromatography

Maybe you found a paper in which they used gas chromatography (GC) to analyze a sample that is similar to yours and now you are wondering if you should try it too. If you are hesitant because you know little about GC, then this article will help you with that. You may already know that…

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How to Choose Your MALDI (Soul) Matrix

gif by Kronin Okay not talking about that kind of matrix. But the kind of matrix that is crucial for MALDI (Matrix-Assisted Laser Desorption Ionization). I talked briefly about MALDI in my last article, Imaging Mass Spectrometry: The New(ish) Kid on the Block. Now I want to tell you more about MALDI, specifically the matrix.…

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Thin Layer Chromatography: The Basics

Like most other chromatographic techniques, thin layer chromatography (TLC) separates out individual compounds from a mixture depending upon the polarity of each compound. The solvent system travels up a silica plate by capillary action and passes over the sample that you spot onto the plate. As the solvent travels up, it moves the compounds present…

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The Many Uses of Thin Layer Chromatography

While working as biologists, we often come across mixtures of compounds, and the first question that strikes our minds is ‘what are the components in this mixture?’ One might think of using chemical assays to find the presence of specific compounds. But that sounds painful, doesn’t it? Well, the good news is that thin layer…

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What Can NMR Do For You – Part Three

Welcome to part three of “What Can NMR Do For You” This is an ongoing series in which we describe how you can use NMR experiments to characterize your protein of interest. In part one, we explained some critical points when doing one-dimensional (1D) NMR experiments on proteins and nucleic acids (e.g. sample preparation and…

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How You Can Use HPLC In Your Research

If you’re an HPLC guru, then you probably think that everyone should be using HPLC.  And you might have a point – HPLC is very powerful and has broad applications across many fields.  But it isn’t the answer to every problem. HPLC (high-performance liquid chromatography) is used to separate mixtures of compounds based on their…

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The Seven Deadly Sins of HPLC

If you work with an HPLC, then you know the frustration of going to use the machine and finding it in disarray. If you’re new to using an HPLC, then the machine can be intimidating to use and you might not know the ins and outs of using it. Here’s an article that has a…

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Running You Through HPLC: How Does it Work?

Your advisor tells you that he wants you to use HPLC to analyze your compound. You know that you’ve heard of this technique before, but you can’t remember what HPLC stands for, let alone how to go about doing it! We’ve all been there, though. Fear not! In this article, we will remind you about…

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What Can NMR Do For You? — Part Two

Welcome to part two of “What Can NMR Do For You?”, a three-part series in which we see how you can use simple NMR experiments in your research. In part one, we went over some key points to keep in mind when doing NMR on proteins and DNA, such as sample preparation, and saw how…

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What Can NMR Do For You? – Part One

Having done my PhD work in an NMR lab, I sometimes feel like NMR doesn’t get the credit it deserves. As a structural tool, it always seems to play second fiddle to crystallography, and not many people seem to know about all of the different types of information that NMR can give you. So why…

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An Introduction to Tandem Affinity Purification

Tandem affinity purification is a development of existing techniques for purifying protein complexes from cells in physiological conditions. It was first described over ten years ago and has become a commonplace laboratory tool. In this brief article I’ll introduce the basic technique and describe some of its advantages. Biology is a team game. Most biological…

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How Does Mass Spectroscopy Work?

Mass Spectrometry, also referred to as mass spec, is an analytical technique becoming increasingly important in bioscience research.  This article will introduce you to this technique, how it works, and how it could be useful to you in your research.  So let’s get started with your introduction to mass spectroscopy… What Is Mass Spectrometry? In…

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