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A CRISPR Approach to Immuno-Oncology
…to overcome these limitations. Ex-Vivo Editing of T Cells as a Therapeutic Strategy The primary challenge to using therapeutic antibodies is the constant deactivation of the immune response by cancer…
Read MoreAn Easy Way to Start Using R in Your Research: Exploratory Data Analysis
…I recommend using ?command to look into details of whatever command you are using. Next time, we’ll learn how to load datasets—from files, from some of the standard ones provided…
Read MoreHow Using Oil Immersion Microscopy Can Increase Your Resolution
Using oil immersion microscopy can help increase your resolution in light microscopy. This, in turn, can lead to better images—and who doesn’t want nicer microscopy images? But, do you know…
Read MoreThe Essential Guide to In-Cell Westerns
…mTORC1 signaling using the In Cell Western technique. Assay Drug Dev Technol 8(2):186–99 Disch JS et al. (2021) Bispecific Estrogen Receptor α Degraders Incorporating Novel Binders Identified Using DNA-Encoded Chemical…
Read MoreHow to Measure Molecular Weight Using Size-Exclusion Chromatography (SEC)
…in the process? Then stick around as we tell you how to calculate sample molecular weight using size-exclusion chromatography. How Does Size-Exclusion Chromatography Work? Let’s go over the basics of…
Read MoreBetter Plasmid Purification: 11 Reasons Your Plasmid Yield is Low
…over some reasons why you might get low yields when you prep plasmids and what you can do about it. 1. Problematic Inserts You prepped two plasmids simultaneously using the…
Read MoreA Beginner’s Guide to How Blunt-End Cloning Works
…already present, by using T4 polynucleotide kinase or using PCR primers synthesized with a phosphate group on the 5’ terminal nucleotide. Table 1 shows the phosphorylation status of inserts and…
Read MoreChick Embryo: A Model System for Studying Angiogenesis
…best. Gently remove the shell using sterile forceps. Once you open it, you’ll have access to the CAM (vasculature). Choose the region of the blood vessels, and using a sterile…
Read MoreHow to Start Using Coding to Automate Image Analysis Part 2: Batch Processing Multiple Images
…work – smarter spreadsheets & databases using VBA, advanced data manipulation using R, Matlab or SPSS, or revamp your blogs & webpages using JavaScript. Finally, just remember this: it’s software,…
Read MoreOverview of Protein Turnover using 35S: how to prevent trashy data and uh-oh moments
…radioactivity using a TCA precipitation is a complete waste of time in my book. You can tell after the wash if incorporation worked using the Geiger counter, and again, this…
Read MoreExcelling With Excel: Analytical Method Validation Using LOD and LOQ
…importance in method validation, and finally a quick method to calculate these values using Excel. What Are LOD and LOQ? LOD and LOQ mean exactly what these terms suggest. They…
Read More11 qPCR Papers Every Researcher Should Know
…of extracting mRNA vs. total RNA or even using crude lysates. Measuring the quality of RNA using methods such as the Agilent bioanalyzer and selecting your RT priming strategy and…
Read MoreXeno Nucleic Acids: Essential Tools in Synthetic Biology and Your Research
…good therapy! You can, however, conduct SELEX using XNAs, and the ligands incorporating these will be much more stable. In one study, an aptamer using XNAs containing hexitol instead of…
Read MoreImaging and Analyzing Your Wound Healing Assay
…are good enough to track gap closure over time. In some specialized cases, fluorescent labelled dyes for tracking two different cell populations or using a transgenic labelled cell line to…
Read MoreAvoid These Pitfalls: Seven (Not So Deadly) Histology Sins
…before using a coverslip. Note: Some advanced objectives have a corrective collar, which can compensate for various coverslip thicknesses. 6. Using 10x buffer This is especially important if you are…
Read MoreMultiplex CRISPR Gene Editing: What You Need to Know
…using a dual-paired gRNA approach (i.e., two pairs of gRNAs that flank the targeted genomic region). [3, 4] However, you might experience diminishing returns when using more than four gRNAs,…
Read MoreMake Better Figures Faster Using Illustrator
…guide to using Illustrator for scientific figures the following sites might be useful: https://www.mmc.med.ed.ac.uk/useful-info/illustrator/ https://www.virology.wisc.edu/acp/tutorials/SciIllustration.pdf Let us know if you have any other quick tips for using Illustrator, or if…
Read MoreFlow Cytometry Gone Viral: Introducing Flow Virometry
…you can enrich the virus populations by using magnetic nano-beads coupled with virus-specific antibodies. However, one caveat when using nano-beads is that multiple viruses might be attached to one bead,…
Read MoreThree-Dimensional Scanning Electron Microscopy for Biology
…have been produced on an ultramicrotome. Serial block face SEM captures images of the sample between removing slices using a diamond knife. Focused ion beam SEM removes slices using an…
Read MoreHow to Choose the Correct Reverse Transcription Method
…oligo-dTs may not give the same result as priming using random hexamer primers. Therefore, you should use the same conditions for each RT reaction. In addition when using SYBR chemistry…
Read MoreAn Easy Way to Start Using R in Your Research: Making Pretty Plots With ggplot
…would use identical code and I figured it was easier to keep using the data we had been using. Of course, all these plots can be ‘prettified’ just like I…
Read MoreHomology Modeling in the World of Proteins
…to align your target and template protein sequences using a sequence alignment tool such as CustalW. [11] This is a very important step in homology modeling because using an appropriate…
Read MoreLevel Up Your Drug Screening With CRISPR
…(Cas9) is a well-established system that manipulates individual genes in a targeted manner using guide RNAs (gRNAs). Scaling CRISPR methods through the creation of gRNA libraries enables large genome-wide screens…
Read MoreHow RNA-FISH Can Complement qPCR, and Vice Versa
…DNA (cDNA) using a reverse transcriptase. This is followed by qPCR using fluorescent probes to measure and quantify the amplification of the target sequence. The Benefits of Using RNA-FISH and…
Read MoreSpinning Around: Tips and Tricks for Using Centrifugal Filters
…initially and then, using a pipette, gently mix the sample 2-3 times to avoid blocking the membrane to avoid losing your hard work and money at this stage. When mixing,…
Read MoreQuantifying & Assessing RNA: TE or not TE?
…Tris-EDTA (TE) buffer to dissolve (or elute, if you are using column purification) RNA. When I was trained in assessing RNA using UV spectrophotometry, graduate students just shrugged at me…
Read More8 Tips to Improve Your Research Reproducibility
…the person using them is consistent and knows what they’re doing. Research is similarly more helpful if it is reproducible, and for several reasons: Lab mates come and go, and…
Read MoreCell Proliferation Round 1: Using Thymidine Analogs With Flow Cytometry
…There are standard ways of doing this using either strong acid or alkali, heat or enzymatic treatment. What can BrdU do for you? Using BrdU, you can more easily identify…
Read MoreUsing Synthetic DNA For Long Term Data Storage
…Yes, you got that right. Think about how nature evolved a long-term storage solution for us to pass down genetic information. Storing data using DNA might sound like science fiction,…
Read MoreExplained: Sensor Chips for Surface Plasmon Resonance and Other Applications
…two most widely used methods — covalent coupling- and affinity-based capture. Covalently Coupled Biosensor Chips Covalent immobilization of the ligand or target is carried out using the amine, thiol, aldehyde,…
Read MoreUsing Gage R&R Studies to Measure Precision in the Lab
…using Gage R&R studies. The degree of variability in measurements is arguably just as important as the result itself. With so many factors that can alter or impact the “trueness”…
Read MoreWhat is Volume Electron Microscopy and Should You Use it?
…using an ion beam to remove sample material affords a much higher resolution in the z direction when compared to using an ultramicrotome. The z-direction resolution can be as high…
Read More7 Tips for using Magnetic Beads for DNA Cleanup
…including regularly calibrating your pipettes. Pay Attention to Your Magnetic Stand Make sure you choose a magnetic rack appropriate for what you are using—whether you are using 8-strip tubes, a…
Read MoreRespect the Ultra
If a random sampling of my hallway is any indication, then half of you don’t think too much of using an ultracentrifuge, while the other half of you are scared…
Read More5 Protein Crystallization Seeding Techniques for Bigger and Better Crystals
…biology and, often enough, the pathology of organisms. And most protein structures are solved using X-ray crystallography, which requires high-quality single crystals of the target proteins. Generating protein crystals is…
Read MoreHPTLC: Basics and Instrumentation
…on the plate using a Derivatizer. Finally, the compounds are quantified by scanning the plate using a scanner. Let’s delve into each of these instruments in detail. HPTLC Instrument Parts:…
Read MoreUsing Flow Cytometry for Fluorescence Resonance Energy Transfer
A marriage of sorts Fluorescence resonance energy transfer, or FRET, is often done using a microscope, which means it can be difficult to analyze large numbers of cells in one…
Read MoreMaking a Mark: How to Set up Your Wound Healing Assay
…to fit different experimental objectives. The wound or gap can be created using a pipette tip or needle, a common inexpensive laboratory tool. This method is appropriate for studies on…
Read MoreHow to Perform DNA Extraction from Dried Blood Spots Using Chelex Resin
…isolating DNA from such samples using BT Chelex 100 Resin3 is described below. Step One: Erythrocyte Lysis Cut out a dry blood spot and place it into a microcentrifuge tube…
Read MoreAn Introduction to Live Cell Imaging
…be avoided by implementing some simple but effective measures. This includes (1) using fluorophores with lower energy (longer wavelength) excitation spectra, (2) using the lowest possible light intensity and the…
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