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Search below to delve into the Bitesize Bio archive. Here, you’ll find over two decades of the best articles, live events, podcasts, and resources, created by real experts and passionate mentors, to help you improve as a bioscientist. Whether you’re looking to learn something new or dig deep into a topic, you’ll find trustworthy, human-crafted content that’s ready to inspire and guide you.
…delaying your research and any actions on its outcomes. This article introduces the different NGS options and their applications and explores some of the commercial resources available for streamlining NGS,…
…have the best chance of a successful and safe organ transplant. Challenges to NGS Becoming a Diagnostic Tool Despite reductions in cost, there are still several challenges to NGS becoming…
…grant trifecta and bought NGS instrumentation, the chances are that you will need to complete your NGS study with the services of a specialized core facility or an outside company….
…an entire NGS project on Partek Flow. Before the NGS bioinformatics even begins, you will likely need to transfer the raw sequencing data files from the server that performed the…
It was not long since the commercialization of NGS (a little more than ten years ago) that scientists went beyond the basics and got creative with the new technology to…
…expression by converting mRNA to cDNA and counting the number of transcripts observed from each gene. For this NGS Channel, we will define NGS as a technology which simultaneously determines…
Rapid genomic analysis offered by next generation sequencing (NGS) is ideal for personalized medicine approaches to clinical genetics, microbiological profiling, and diagnostic oncology. Many standard clinical samples are preserved as…
…you can ensure that your NGS results are robust. No one wants to publish findings that other scientists fail to replicate, but unfortunately it happens all the time. If you…
…to create multiplex pools, and flow-cell or PGM-chip loading. I’m going to focus on the last two in this article as there are many ways to quantify NGS libraries but…
DNA sequencing (PCR, Sanger or next-generation sequencing (NGS)) is a now familiar part of any molecular biology lab. But ‘RNA-seq’, the so-called “Cinderella of genetics”, is now becoming the belle…
…throughput sequencing methods, also known as next generation sequencing (NGS), SNPs can be identified in the large amounts of DNA sequence that is generated. There is some research being done…
Making a Next Generation Sequencing (NGS) library can seem a bit daunting to the new user, as failures can be expensive. But don’t be put off, as NGS library preparation…
Next-generation sequencing (NGS) has ushered in a new era of understanding of both the inner workings and the function of the genome. NGS allows researchers to look at traits—including diseases—that…
…next generation sequencing (NGS) experiment does not look good. You panic and, perhaps, let loose a scream of frustration—aaarrrrggghhhh! This project was going to be your primary data source for…
Size Selection via Gel Electrophoresis Whether you are using NGS for whole genome sequencing, SNP variant analysis, HLA typing, HLA matching, or even transcriptome or miRNA analysis by RNA-seq, size…
The completion of the Human Genome Project in 2003 ushered in a new era of rapid, affordable, and accurate genome analysis—called Next Generation Sequencing (NGS). NGS builds upon “first generation…
Used for matching organ transplants to donors and other applications, human leukocyte antigen (HLA) typing is rapidly shifting from older methods to NGS technologies. This is a major step forward,…
Next-generation sequencing (NGS) really has taken the world by storm! In NGS, millions of short ‘read’s are sequenced in a short space of time, leaving you with vast amounts of…
…can use the on an NGS platforms, such as Illumina and Ion Torrent AMP-NGS in the Clinic. AMP-NGS in the Clinic Hot-spotting point mutations is frequently used in…
NGS is not a three-headed monster. However, it can be a difficult concept to grasp—especially when you are getting started. There is a lot of new terminology, and a whole…
Construction of high-quality sequencing libraries is pivotal to successful NGS, and DNA quality is one of the most critical aspects of library preparation. As this Nature Methods paper illustrates, DNA…
…but equally some genomes are very GC biased and some NGS applications also introduce a strong GC bias (such as Bis-seq). Duplicate Sequences. This plots the number of times the…
…those processing NGS data who have not been privileged to earn a computer science degree. Tools such as Galaxy are helping to bridge the gap between computer science and biology….
…tale of three next generation sequencing platforms: comparison of Ion Torrent, Pacific Biosciences, and Illumina MiSeq sequencers. BMC Genomics. 13:341. The Molecular Ecologist’s 2014 NGS Field Guide. Next generation sequencing…
…scalable. A hub for innovation. But what exactly is NGS and how can it help your research? In these next few articles we will be discussing what NGS is, the…
…you can examine and requires prior knowledge of gene sequences, meaning identification and profiling of novel genes is not possible. One solution is to utilize next-generation sequencing (NGS), which can…
…sample and template preparation kits that you can purchase at PACBIO to speed things up. 2nd Gen NGS v.s. 3rd Gen NGS So by now you are probably wondering how…
…of fusion genes in cancer. Quality Metrics The standard quality control metrics for all NGS runs, which can be derived from the runtime software and from FASTQC include: total number of…
In my previous article ‘Choosing a scripting language for next gen sequencing: Python, Perl, and more’ I discussed several of the more common programming languages used for next generation sequencing…
“Any sufficiently advanced technology is indistinguishable from magic.” – Arthur C. Clarke In the fast-moving field of next generation sequencing, standard practices are evolving rapidly. Today, more and more labs…
The Irish Famine (or ‘Great Potato Famine’ if you live outside the Emerald Isle) killed one million people and forced another million to leave the country between 1845 and 1852….
Of all the sample prep steps necessary for next generation sequencing, DNA size selection may have the greatest impact on quality of results. After all, ineffective sizing can waste sequencing…
…Antibody or affinity-based enrichment Methyl-sensitive restriction enzymes With the advent of Next Generation Sequencing (NGS), these principles have been integrated in its platforms, to get a large set of data…
…run remain high for NGS approaches. The continued advancement of sequencing technology and falling costs suggests that NGS will soon become the dominant validation option for CRISPR editing. Enzyme Mismatch…
…of proteins influencing their function. This article will show the known roles of ncRNA in regulating transcription and demonstrate how next-generation sequencing (NGS) techniques have the potential to discover new…
…on learning Python 3. Applications of Python in Biology If NGS is your platform, then check out these earlier Bitesize Bio pieces on choosing the right language for NGS and…
…generation sequencing (NGS) technology requires the DNA polymerase to copy a long stretch of DNA (sometimes up to 50kb) as NGS is churning out genetic codes at a lightening speed….
…pipeline to facilitate data analysis is a critical step in analyzing NGS data. There are some concerns prior to the analysis that should be taken into consideration: Run quality metrics…
RNA-seq is based on next-generation sequencing (NGS) and allows for discovery, quantitation and profiling of RNA. The technique is quickly taking over a slightly older method of RNA microarrays to…
Size selection is a critical step in NGS pipelines, but may be most challenging for studies of small RNAs. The concept behind size selection is simple: separate a sheared DNA…
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