Search results for: CLEM

Cryofixation and Chemical Fixation for Electron Microscopy

Published April 23, 2019 |

Researcher analyzing electrom microscopy images highlighting two methods of fixation for electron microscopy

…lead to protein denaturation, which may be problematic in downstream antibody-labeling techniques. This is a challenge when using correlative light and electron microscopy (CLEM). There are several chemicals that are…

What Is Cryo-Electron Microscopy? A Brief Introduction

Published April 26, 2022 |

A graphic of a fist punching the air to describe the concept of what is Cryo-Electron microscopy

…Microscopy There are several sub-techniques within cryo-EM. These are: Scanning electron cryomicroscopy (cryo-SEM). Cryo-correlative light and electron microscopy (cryo-CLEM). Cryo-electron tomography (cryo-ET). Transmission electron cryomicroscopy (cryo-TEM). And check out Figure…

Electron Microscopy Sample Preparation: 10 Pieces of Expert Advice

Published June 1, 2023

…particularly costly in a CLEM workflow. Careful planning of your experimental design before you obtain your samples will help ensure you use the right preservation technique. “…the solution is always…