Tips and Tricks for Western Blotting and Antibody Selection

You will learn:

  • How to overcome common Western blot issues
  • Which criteria to use for antibody selection
  • Useful information for your grant preliminary data

Western blotting is an important and widely used technique in cell and molecular biology. A Western blot is often used in research to separate and identify proteins based on molecular weight and detection by labeled antibodies. Even though this procedure is simple and very common, many problems can arise, leading to unexpected results. Sample preparation, antibody selection and blocking buffers are a few considerations, but there are many.  This webinar will give you an overview of the techniques then focus on tips and tricks that will help you get the results you want for your projects, publications and grants.