Getting Correct Color from Your Camera

Jerry Sedgewick

In this webinar you will learn:

  • Proper and ethical post-processing of microscopy images.
  • The importance of gamma in microscopy images.
  • How to properly control and compensate for the factors affecting color reproduction.

Abstract:

Seeing is believing—or is it? When a flash doesn’t go off, a white wedding dress turns yellow. Do you believe it?

Seeing and believing that the dress is yellow may not be that important for the casual wedding image, but in microscopy imaging seeing and believing is critical. Microscopy images that contain color, such as images from histology­-stained specimens, must be compared and analyzed. If the color reproduction is poor, then there’s a chance that important information will be overlooked, not seen, and not believed.

There are a number of factors that affect color reproduction. The light source, camera type, and white balance all play a role—even the choice of monitor can affect color reproduction. These aspects all must be optimized for the particular type of microscopy imaging being performed. Here we discuss these factors and how to ensure that the image you see down the eyepiece matches what you see on the screen.