Posts Tagged ‘how to’
How To Fix Adherent Cells For Microscopy And Imaging
Adherent cell fixation is a crucial step in preparing cells for microscopy and imaging, ensuring that cellular structures are preserved for detailed analysis. Read our 8-step guide on how to effectively fix adherent cells to your microscope slides, including tips on sterilization, coating, and fixation methods, right here.
Read MoreTips for Using and Maintaining Your Laboratory’s Freeze Dryer
Keep your lab’s freeze dryer running smoothly with our expert tips on care and maintenance, including oil changes and vacuum leak fixes.
Read MoreHow to Write a Thoughtful Scientific Discussion Section for Your Paper
Having trouble writing the discussion section for your scientific paper? Help is at hand. Read our do’s and don’ts to help you have your paper polished up in no time.
Read MoreThe Essential Guide to In-Cell Westerns
In-cell Westerns are a powerful technique that has enhanced how researchers analyze protein expression levels and signaling pathways within fixed cells. Learn about their primary advantages, applications, and some of the best tech and products to perform them.
Read More5 Ingredients for the Perfect Protein Purification Buffer
Purified proteins must be kept soluble and active. Discover how to design the perfect protein purification buffer—from additives you can include and their appropriate concentrations to important considerations.
Read MoreWhat Happens If You Remove the Word ‘because’ from Your Vocabulary?
By removing the word ‘because’ from your vocabulary, you can gain a newfound sense of freedom, creativity, and authority in your decisions. Learn to make choices without justifications and experience the world with a fresh set of eyes.
Read More8 Tips to Improve Your Research Reproducibility
Performing reproducible research is critical in science. This article discusses eight steps you can take to make your research more reproducible.
Read MoreElectron Microscopy Sample Preparation: 10 Pieces of Expert Advice
Do you prepare samples for electron microscopy and want to save time, money, effort, and frustration? This article provides hands-on advice to help you get the best possible data out of your EM experiments.
Read MoreA Beginner’s Guide to Protein Structure Prediction
Dive into the world of protein structure science, where AI is revolutionizing the field by offering accurate predictions and free, open-access tools. In this beginner’s guide, we’ll explore these groundbreaking tools, discuss their strengths and weaknesses, and examine the implications for traditional experimental methods like NMR and X-ray crystallography.
Read MoreAn Easy and Reliable Nuclear Extraction Protocol
Here’s your reliable and adaptable nuclear extraction protocol. It contains buffer recipes, explanations of what the reagents do, and top tips to boost yields.
Read MoreMaximizing Protein Extraction from Yeast Cells: A Comparison of 4 Methods
Confused about yeast protein extraction methods? Let this article guide you through the process and help you select the most appropriate method based on your research goals and available resources.
Read MoreHow to Keep Your Centrifuge Alive: 5 Easy Tips
The more expensive your lab centrifuge, the more sensitive and the easier it is to break. What can we do to give these pricey monsters a long, successful tenure in the lab? Read our 5 easy tips.
Read MoreHow to Use a Fume Hood: 10 Top Tips
Want to know how to use a fume hood? Keep your head out of it for starters. Here are 10 extra tips to keep you safe when using one!
Read MoreAre Your Patterns Holding Your Research Back?
We all have patterns for tasks. While good patterns are helpful, bad ones can sabotage your career. Learn to identify and destroy harmful patterns.
Read MoreHow to Conduct a Risk Assessment: 6 Essential Steps to a Safer Lab
It’s crucial that we stay safe in the lab. To prevent accidents, we should risk assess our work. So here’s how to conduct a risk assessment in 6 steps.
Read MoreHow to Handle Bases in the Lab: Everything You Need to Know
We all know acids are dangerous chemicals, and you should handle them carefully. But how should we handle bases? Read on for top tips for handling bases safely in the lab.
Read MoreHow To Passage Cells in Culture
Learn the basics of how to passage cells in culture with this easy-to-follow guide. While the specifics change per cell line, the basics remain.
Read MoreUnderstanding Cell Passage Number and How to Calculate it for Cell Cultures
Not sure what we mean by cell passage number? Confused about how to calculate it? Wondering if there is a maximum number? We explain it all.
Read MoreHow to Measure Molecular Weight Using Size-Exclusion Chromatography (SEC)
We often use size-exclusion chromatography to purify samples. Read this article to learn how to use it to measure sample molecular weight as well!
Read MoreGoing Serum-Free: The How and Why of Removing Serum From Your Media
While using serum in cell culture can keep your cells healthy and happy, there are some notable downsides. We discuss the pros and cons of using serum in cell culture media and how to remove it if you want to eliminate it from your workflow.
Read MoreHow to Perform RNA Quality Control in the Lab: 3 Key Considerations
Discover what RNA quality control is, why it’s so important for your experiments, and how to undertake it with these 3 key considerations.
Read MoreHow to Read a Scientific Paper
We show you how to read a scientific paper efficiently to make the most of your reading time.
Read MoreJournal Club: How to Prepare Effectively and Smash Your Presentation
Journal Club. So much more than reading a paper aloud. So many ways to mess it up. Got to present one? Then read our journal club toolkit.
Read MoreMultiple Fragment Ligation: The Why and How
You may be familiar with standard single fragment ligations, but did you know you can ligate multiple fragments into your vector all at the same time! Discover how to perform multiple fragment ligation, including the different methods and troubleshooting tips for when things go wrong.
Read MoreTake Care of Your Tools: Cleaning Pipettes
Contamination in your pipette can mean contamination in your experiments, making cleaning pipettes regularly critical. Here’s an easy guide on how to do it.
Read MoreWhat is Touchdown PCR? A Primer and 5 Easy Tips For Success
Got non-specific PCR amplification? You need touchdown PCR! Discover what it is, how it works, and get 5 top tips for performing touchdown PCR.
Read MoreHow to Use a pH Meter Correctly in 4 Simple Steps
A neglected pH meter means less reliable experiments, poor reproducibility, and your time wasted. So, learn how to use a pH meter correctly!
Read MoreHow to Handle Strong Acids in the Lab
Acids are common and essential chemicals in any research laboratory. In this article, you’ll get advice on how to handle strong acids safely to give you the confidence to routinely prepare, handle, and dispose of them in the lab.
Read MoreHow to Check the Accuracy of Your Pipette in 7 Easy Steps
I’d bet that your trusty pipette is probably one the the most often used tools in the lab. But do you have any idea how to check the accuracy of your pipette? We’ll take you though how to do this in just 7 easy steps.
Read MoreThe qPCR Standard Curve: The Key to Good qPCR Data
Can’t be bothered doing a qPCR standard curve? Find out why it’s important to getting great results and how to do it.
Read MoreThe Basics of NMR Part 3: Multidimensional NMR
NMR is a powerful structural tool for studying proteins. Learn how to use multidimensional NMR to identify ligand binding sites and more!
Read MoreHow (and Why) To Manipulate Plasmid Copy Number
Here we take a closer look at plasmid copy number and examine how it can be manipulated in the lab giving you flexibility in your work.
Read MoreHow Confluent Are Your Cells? A Beginner’s Guide to Measuring Cell Culture Confluency
Cell confluency can affect cell behavior and growth. Find out what confluency is, how to measure it, and why it is a crucial consideration for your experiments.
Read MoreThe Basics of NMR Part 2: NMR and Protein Dimerization
NMR can generate tons of useful data for protein samples. Learn all about NMR and Protein Dimerization in this easy intro.
Read MoreCreativity in Science: How a Good Imagination Can Help Your Research
It pays dividends to think creatively about your research. Here are 8 tips to supercharge creativity in science and ensure a great environment to help your imagination flourish.
Read MoreTips on Receiving and Applying Constructive Feedback for Scientists
Are you looking to improve your lab work, publication record, and grant success rate? Then read our guide to feedback—what it is, where it should come from, and how to give it to others.
Read MoreWhat Is Cryo-Electron Microscopy? A Brief Introduction
You don’t have to be a genius to understand Cryo-EM. Discover the fundamentals of this powerful microscopy tool and what propelled it into the scientific mainstream.
Read MoreAn Introduction to Circular Dichroism Part 2: Circular Dichroism Practice
Circular dichroism is a type of spectroscopy that can tell you the type and percentage of secondary structure units in a protein sample. Here are 5 handy pointers on best circular dichroism practice to set up your experiment properly.
Read MoreTop Tips for Getting Science Research Grants
Getting science research grants is becoming increasingly competitive and difficult. Read our top tips to maximize your chances of success.
Read More5 Protein Crystallization Seeding Techniques for Bigger and Better Crystals
Protein crystals are crucial for structure solution via X-ray crystallography but are notoriously hard to grow. We’ve got you covered with 5 clever protein crystallization seeding methods to grow impressive protein crystals.
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