We always use 10,000- 13,000 x g for 1-2 minutes in a microcentrifuge.
100,000 RPM might be a bit much.
Bacteria are heavy so they’ll pellet with less force.
I don’t know but I would guess that at 100,000 RPM, it would take about 10 seconds to pellet bacteria- it would take longer than that for the centrifuge to reach that speed.
I agree with Suzanne. To get to 100,000g/rpm, you would need to use a ultracentrifuge, and it takes a significant amount of time to get up and running and then slow down and stop. I would also be worried about damaging the cells at 100,000g/rpm.
If you are using the pellet after this step, you can pellet the cells in 20-30 seconds in a microfuge. You leave more cells in the supernatent than you would if you spun then for 1-2 minutes, but if you are more concerned with speed than you are with quantitative recovery of the cells, then this might work for you.
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