Home Forums General Discussion Maximum Speed to centrifuge bacteria

This topic contains 5 replies, has 5 voices, and was last updated by Avatar of Marcus Marcus 3 years, 10 months ago.

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  • #3989
    Avatar of Marcus
    Marcus
    Member

    Hi,

    Just wondering is there a maximum speed I can spin bacteria. I just need to make a pellet in the shortest time possible but I dont want to damage the cells. Is it damaging at say 100,000 rpm/ 1min?

    Thanks.

    #17812
    Avatar of Suzanne Kennedy
    Suzanne Kennedy
    Participant

    Hi Marcus,
    We always use 10,000- 13,000 x g for 1-2 minutes in a microcentrifuge.
    100,000 RPM might be a bit much.
    Bacteria are heavy so they’ll pellet with less force.
    I don’t know but I would guess that at 100,000 RPM, it would take about 10 seconds to pellet bacteria- it would take longer than that for the centrifuge to reach that speed.

    Suzanne

    #17815
    Avatar of Jode Plank
    Jode Plank
    Member

    I agree with Suzanne. To get to 100,000g/rpm, you would need to use a ultracentrifuge, and it takes a significant amount of time to get up and running and then slow down and stop. I would also be worried about damaging the cells at 100,000g/rpm.

    If you are using the pellet after this step, you can pellet the cells in 20-30 seconds in a microfuge. You leave more cells in the supernatent than you would if you spun then for 1-2 minutes, but if you are more concerned with speed than you are with quantitative recovery of the cells, then this might work for you.

    #17816

    I don’t know how much force cells can handle, but I think the important question should be “How much force do I need?”

    Even if cells can withstand 100k rpm, you probably don’t need that speed. For e.coli in LB, 20 seconds at 13k rpm in a microfuge will pellet the cells well.

    #17823
    Avatar of Gough Au
    Gough Au
    Member

    Cheers folks, I dont know where I was getting 100,000 from I meant 10,000 rpm. They are helpful insights, appreciate it.

    #17828
    Avatar of Pranay Dogra
    Pranay Dogra
    Member

    I agree with Suzanne. Usually centrifuging liquid cultures at 10,000 rpm from 3 – 5 min gives a very sturdy pellet and the supernatant can be discarded easily.

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