Hello everyone, I was thinking to study the FRAP of my protein of interest. Can anyone tell me what are the possible inferences, apart from the protein kinetics, can be extracted out from the FRAP and FLIP results. I am looking forward to here/discuss about this topic from all of you..Thank you.

First of all, English is not my first language so please be patient. Thank you for your understanding. I have a research project about the diversity of soil microbial communities (Bacteria and Arachaea) in a desert ecosystem. My sample were extracted and kept in -80 degrees environment now. PCR will be utilized before sending my [...]

What is the mimimum pI difference in proteins that enables their efficient separation by isoelectric focusing? I have two forms of the same protein with pI difference of less than 0.02. Would it be possible to separate them by IEF?

Background: I\’m in an ecology lab (focus on entomology) where I\’m the first person to actually do the molecular work (instead of distant collaborators) and I didn\’t have time to research DNA extraction methods before my study started. I was told to \”just preserve the samples on a filter in ethanol and extract the DNA [...]

i want to start a work where the first step is the creation of the vector itself.. i have cloned a eGFP fragment in the pUB23 vector and transformed yeasts BY4741 cells. this GFP fusion will be used as reporter protein when i further clone into it my desired peptide fragment and study self degradation [...]

hello everyone, As a fresh Bachelor graduate, i have built up a collection of paper across multiply discipline (ecology, microbiology, biochem & a lot of random topics ><). i read & annoted some of them and used to rely a on \"home-made\" database from Excel, so that i search for them when i need them. [...]

As I know, I can search whether a gene expression or not in the data set UniGene, but it is in tissue. If I want to know what cell line can express this gene, is there any public data sourse I can refer to?

When I was reading about some Enzymes for my research I came across some units like OAA, OCMU, HACU, SKB, ALU, LVU, FU, TU etc., I have not heard about this terms. I want the details regarding enzyme units. Can you please give the details regarding different enzyme assays? is there any method to convert [...]

Hi, I would like to know if contaminants present following an extraction of RNA could affect the yeild of RNA? I have a contaminant in my total RNA extracted from leaf tissue that strongly absorbs at 230nm, possibly a polysaccharide, and I would like to know if this effects my quantification. I am not using [...]