PombeGeek Ph.D. Candidate at University of Vermont about memy commentsmy questions on protein purification from yeast strain, 27th of July, 2011 Have you looked into over-expressing a tagged (GST, etc...) copy of your protein? We routinely use the Gal promoter to control expression of essential proteins in S. cerevisiae for later purification. page 1 of 11
on protein purification from yeast strain, 27th of July, 2011 Have you looked into over-expressing a tagged (GST, etc...) copy of your protein? We routinely use the Gal promoter to control expression of essential proteins in S. cerevisiae for later purification.