At the end of my last article, I began providing some of the tips and tricks of practical work with enzymes at the bench. Now we’ll start to cover one of the cornerstone techniques of enzymology work: the enzyme assay. Starting with the simple and eventually working our way to the more complex, this article [...]

In Part 1 of this series, we began our journey into the fascinating world of enzymology. We looked at the most basic concepts of what an enzyme is and the incredible jobs it can do. In Part 2 of “Working with Enzymes” I will look at some things to bear in mind, to keep your [...]

Bitesize Bio readers who have an interest in enzymology will hopefully remember my recent series on the Basics of Protein Phosphorylation. On the back of that series comes something which is, believe it or not, even more basic- and with perfect timing too, seeing as how Bitesize Bio is launching a webinar series on Chemistry [...]

Chris Dieni wraps up his series on the Basics of Protein Phosphorylation with this final part on further reading and resources available.

Chris Dieni explores the promiscuous yet intriguing nature of protein phosphatases

So far in our “Basics” series, we’ve taken an overview of the major players involved in protein phosphorylation, and some of the tools that one might use to study them. Now, we’ll return to the protein kinases to take a deeper look- though our “deeper” look will barely scratch the surface, as there are currently [...]

In the previous article in this series, we looked at the major players involved in protein phosphorylation: protein kinases, protein phosphatases, and target proteins. This time, we’ll glance over some of the tools that we can use to study various aspects of protein phosphorylation, focussing on a few I’ve personally come across. 1. Tools for [...]

The focus of my grad studies and postdoctoral research has been the analysis of proteins regulated by reversible protein phosphorylation. However, the number of unique facets in which protein phosphorylation can be studied is astounding, and is diverse as any niche of the biosciences. This article is the first in a series that will give [...]

Where did bio begin for you? That intriguing question has been the central focus of an interesting experience I just had. How this all started It began on a Saturday morning. After I woke up and fed my cat, and before making my own breakfast, I went straight to my computer to check which emails [...]