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Data Analysis for Three-dimensional Volume Scanning Electron Microscopy

In recent years, three-dimensional (3D) scanning electron microscopy techniques have gained recognition in the biological sciences. In particular, array tomography, serial block face scanning electron microscopy (SBFSEM) and focused ion beam scanning electron microscopy (FIBSEM) (described in Three-Dimensional Scanning Electron Microscopy for Biology) have shown an increase in biological applications, elucidating ultrastructural details of cells […]

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In Microscopy & Imaging, Zeiss 25th of January, 2017
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3D SEM read on

Three-Dimensional Scanning Electron Microscopy for Biology

Scanning electron microscopy (SEM) is a powerful technique, traditionally used for imaging the surface of cells, tissues and whole multicellular organisms (see An Introduction to Electron Microscopy for Biologists)(Fig. 1). While the resultant images appear to be three dimensional (3D), they actually contain no depth information. However, there are several SEM techniques that can obtain […]

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In Microscopy & Imaging, Zeiss 4th of October, 2016
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The Fast Module for ZEISS LSM 880 with Airyscan: Confocal Superresolution Imaging with Four Times the Speed and Improved Signal-to-Noise Ratio

First introduced in August 2014, the Airyscan detector from ZEISS represents a new detector concept for laser scanning microscopy (LSM) that enables a simultaneous resolution and signal-to-noise (SNR) increase over traditional LSM imaging. The Airyscan detector design substitutes the conventional LSM detector and pinhole scheme for an array of 32 sensitive GaAsP detector elements, arranged […]

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In Microscopy & Imaging, Zeiss 31st of May, 2016 Presented by: Chris Power
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Digital Classroom read on

Producing Your own Digital Classroom with ZEISS: Starring Wi-Fi-enabled Microscopes and Mobile Apps

It’s hard to believe, but microscopy is nearly 400 years old. Since the early 1600s, scientists have sought new and improved ways to view microscopic images, record them and share them with eager students and colleagues. In 1846, major advances in microscope engineering began in the fledgling workshop of Carl Zeiss, culminating with the first […]

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In Microscopy & Imaging, Zeiss 12th of April, 2016
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Microscopy Education In The Digital Age: Upgrade Your University With A Digital Classroom

In this webinar, Silvia Zenner-Gellrich , Ph.D., product manager at ZEISS, will share: How to integrate your microscopes with digital technology How you can use the Labscope imaging app in the classroom The benefits of using connected microscopes in your classroom Abstract: Universities and schools alike are competing which each other for the best rankings […]

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In Microscopy & Imaging, Zeiss 17th of March, 2016 Presented by: Silvia Zenner-Gellrich
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Microscope Maintenance read on

Let There Be Light! Microscope Maintenance Part 2: Köhler Illumination

In Part 1 of these articles, you’ll have learnt about common microscope light sources and how to replace and align these correctly. In this article, we will discuss the importance of Köhler illumination and how to set up the microscope to achieve optimal imaging results. What is Köhler illumination? Before discussing this technique, let us […]

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In Microscopy & Imaging, Zeiss 9th of March, 2016
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Microscope Maintenance read on

Let There Be Light! Microscope Maintenance Part 1: Routine Care and Replacing Bulbs

Do you want the best imaging experience each time you use a microscope? Well, this is a rhetorical question, as we all desire that these delicate optical instruments are clean, free from immersion oil and correctly aligned. From the routine checking of slides and, more importantly, for capturing images for presentations and publications, microscopes need […]

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In Microscopy & Imaging, Zeiss 24th of February, 2016
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Enabling Connectomics with Multi-beam Scanning Electron Microscopy – The World’s Fastest SEM Just Got Even Faster

In this webinar, presented by Anna Lena Eberle, PhD, Neuroscience Specialist and Product Manager for ZEISS MultiSEM, you will learn: Challenges and relevance of 3D imaging for Connectomics 3D volume imaging with scanning electron microscopy methods Newest technologies for ultrastructural high-throughput imaging Abstract: Recent years have witnessed major progress in three-dimensional (3D) microscopy techniques for […]

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In Microscopy & Imaging, Zeiss 12th of November, 2015 Presented by: Anna Lena Eberle, PhD
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ZEISS Airyscan: A Brave New Microscopy World with Sharper Confocal Resolution

Confocal laser scanning microscopy is today’s standard method of obtaining detailed 2-D and 3-D information at the cellular level. The imaging technology has become popular in biomedical research because of its ability to produce precise, optically sectioned images, and address many types of samples and application demands. Over the past 25 years, improvements have been […]

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In Microscopy & Imaging, Zeiss 30th of September, 2015
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How to Troubleshoot Problems with Fluorescently Tagged Proteins

Using fluorescent proteins as imaging probes is a widespread and versatile technique in microscopy. You can use them in a wide range of living systems, from single cultured cells to complete organisms and animals. Fluorescently tagged proteins can be used to track and examine real-time localization, interactions and translocation of your protein of interest, as […]

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In Microscopy & Imaging, Zeiss 5th of May, 2015