yevgeniy

Yevgeniy grew up in New York, but decided to transplant himself to the West Coast for his PhD studies at the Scripps Research Institute, where he studied mechanisms of gene regulation in the immune system. Recently, Yevgeniy again found himself in New York City, pursuing a post-doctoral research project in oncology. Realizing that his true passion was writing, Yevgeniy decided to leave academia and pursue science writing as a freelancer to share his passion for fostering communication between the scientific community and the public. In his spare time Yevgeniy works as a Krav Maga self-defense instructor.

Articles by yevgeniy:

Cell Counting with a Hemocytometer: Easy as 1, 2, 3

Many biological applications such as microbiology, cell culture, blood work and many others that use cells require that we determine cell concentration for our experiment. Cell counting is rather straightforward and requires a counting chamber called a hemocytometer, a device invented by the 19th century French anatomist Louis-Charles Malassez to perform blood cell counts. A…

08 Dec 2014 Cells and Model Organisms

Interview Techniques: Interview like a STAR

During your time interviewing for different jobs, more likely than not you will encounter employers who conduct behavioral interviews. What is a behavioral-based interview, you may ask? Behavioral interviewing is supposed to uncover your past job-related behavior to predict how you will behave in the future. It is based on the assumption that your past…

14 Oct 2013 Career Development & Networking

Mycoplasma: The Hidden Anarchist of Cell Culture

It is the black death of cell culture. Scientists don’t dare utter its name and many a graduate student has fallen victim to its indiscriminate menace. These stealthy anarchists infiltrate quietly but deliberately until their numbers swell and then they attack in strength, overwhelming their victims before they can put up a fight! What is…

04 Sep 2013 Cells and Model Organisms

5 Ways to Really Screw Up Your RNA Prep

Unlike DNA, which can last for eons (if stored correctly!), RNA is a fragile and degradation-prone cousin. After working with RNA for a while, one becomes quite paranoid about handling RNA because even a single sneeze or drop of saliva can potentially affect your results. The reason, which was discussed earlier, is that there are…

29 Jul 2013 DNA / RNA Manipulation and Analysis

Crush Like an Elephant, Soak Like the Rain: Old-School DNA Gel Extraction

In my previous article on DNA gel extraction, I explained how most commercially available DNA gel extraction kits work. However, there was a time before our society was blessed with these convenient marvels of technology and scientists had to summon the gods of “Crush and Soak”. This method has been proven for millennia, as people…

07 May 2013 DNA / RNA Manipulation and Analysis

How DNA Gel Extraction Works

Isolating pure DNA is key to many downstream applications for molecular biologists.  Isolating large quantities of pure DNA used to be a laborious task.  But thanks to commercially available kits, older methods have been streamlined to allow efficient recovery of pure DNA. In this article, I will talk about a method called DNA gel extraction,…

01 May 2013 DNA / RNA Manipulation and Analysis

The A-Z List of Things that go “Missing” in the Lab

Here is a fun list of things that you are most likely to lose to light-fingered colleagues or nocturnal ghosts of academia. The emphasis here is on fun, so as disclaimers often go, if your experience proved somewhat different, this list “does not represent the actions of every individual or ghost who you might encounter…

01 May 2013 Organization & Productivity

Blast your way to quicker, cheaper bacterial transformations

If you want a more efficient, cheaper way to do bacterial transformation, if using a small DIY cannon to create a shockwave that forces bacteria to take up DNA from its surroundings sounds like your type of fun (if not, why not?!), you are definitely going to like this article. In a study published in 2011,…

08 Apr 2013 DNA / RNA Manipulation and Analysis

Isolation of total RNA, including microRNA, from cells and tissues

Whereas DNA can survive for millennia, RNA is short-lived, which is a bummer if you are trying to isolate it. The reason – as we discussed recently – is that RNA is prone to degradation by enzymes called RNases. Therefore, isolation of total RNA from cells and tissues requires a method that will efficiently isolate…

19 Mar 2013 DNA / RNA Manipulation and Analysis

Outgrown the Roost: Passaging Suspension Cells

Previously, you have learned about passaging adherent cells and read a quick protocol to make it happen. In this article, I will talk about passaging suspension cells. Some cells naturally live in suspension in body fluids and do not attach to surfaces, such as cells of hematopoietic origin found in our bloodstream. Culturing these suspension…

06 Feb 2013 Cells and Model Organisms