Vicki Doronina

Vicki did her PhD in Molecular Biology at the University of Edinburgh. She had been working as a postdoc in several Russel group UK universities, while honing her skills in scientific and creative writing. She is now a pen for hire. Check out my proudest achievement, which may be useful for you: The BiteSizeBio Guide for Protein expression

Articles by Vicki Doronina:

Top Ten Tips for TAP

Tandem affinity purification (TAP) is a versatile technique allowing the isolation of proteins for various purposes including Western blot and mass-spectrometry. The target protein is fused with protein A from streptococcus and the calmodulin binding domain, which together comprise the TAP-tag (for an introduction to TAP-tagging, see this article). To purify a TAP-tagged protein from…

14 Nov 2012 Protein Expression & Analysis

Book Review: “The Double Helix”, by James Watson

Is it just me, or are there not many well-written scientific memoirs around? Even the words “scientific memoir” brings up an image of a long and boring book. There are a lot of good books written about scientists, but not by scientists. Maybe it’s because the scientists are trained to write logically, objectively and dispassionately:…

19 Oct 2012 Inspiring & Thought Provoking

DEPC: The Wicked Witch of RNA?

If you have ever worked with RNA, you know about DEPC (diethylpyrocarbonate). You add it to water at a concentration of 0.1%, shake or stir, incubate at 37°C for two hours or at room temperature overnight and, as if targeted by a magic bullet, the RNAses that may have been in the water are gone.…

10 Oct 2012 DNA / RNA Manipulation and Analysis

How To Handle A British Supervisor: A Foreigner’s Guide

The United Kingdom, formerly known as Great Britain, has a long scientific tradition. British academic institutions are among the best in Europe and possibly the world (there is a potential conflict of interest here: while the author is not British by birth, she has spent many years studying and working in the UK). It is…

26 Sep 2012 Dealing with Fellow Scientists

How to Amplify Difficult PCR Substrates

During my postgraduate studies, I did literally one PCR reaction with a pre-optimised protocol on a not especially difficult template. So my karma came back with vengeance, when as a part of my first postdoc I had to amplify a template containing a 35 bp-long GC-rich stem-loop, which proved to be extremely difficult. This was…

14 Sep 2012 PCR, qPCR and qRT-PCR

Zero Tolerance: A Perfectionist’s Guide to Aseptic Technique

Arguably, molecular biology is impossible without microbiology – even if you work exclusively with transgenic mice, you may one day need to amplify a vector in E. coli. And microbiology is definitely impossible without good aseptic technique. The main principle of good microbiological practice is a zero tolerance approach: it’s good to be a little…

29 Aug 2012 Cells and Model Organisms

How to Do a Kit-free Midiprep

Commercial kits are supposed to be to homemade protocols what lifts are to stairs: they should work faster and save you physical exertion. However, in many cases, taking the staircase (e.g. the DIY approach) works better in some ways – and it is always cheaper. BitesizeBio has previously published protocols for homemade plasmid minipreps. I’d…

23 Jul 2012 DNA / RNA Manipulation and Analysis

Got Phage? Here’s how to get rid of it.

Summertime… The birds are singing, the trees are growing. Your tissue culture has sprouted yeast contamination, your yeast culture is happily growing bacteria. Your bacterial culture was growing calmly and predictably, dividing every twenty minutes, but suddenly its optical density has dropped, and it’s full of some sort of filaments and clumps. Or you did…

18 Jul 2012 Cells and Model Organisms

How to Be The Lab Bastard

We all know them. You might even be one. The Lab Bastard is the one who considers himself (or herself!) superior to all other mere mortals in the lab. He would never degrade his talent by doing communal jobs in the lab, but swans around, absolutely sure that his experiments are most important and his…

03 Jul 2012 Dealing with Fellow Scientists

The Easiest Yeast Transformation Protocol on Earth

There are several yeast transformation protocols around, and most of them require a lot of steps: overnight starter culture, dilution and growth to logarithmic phase, several washes, and so on… These protocols work very well since they have been optimised for maximum transformation efficiency, which is needed for applications like library construction. But they are…

22 Jun 2012 Cells and Model Organisms